PDPK1 Rabbit Polyclonal Antibody

CAT#: TA392893

PDPK1 (phospho-S241) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
• Specificity: p-PDPK1 (S241) polyclonal antibody detects endogenous levels of PDPK1 protein when phosphorylated at Ser241.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 63 kDa
• Gene Name: 3-phosphoinositide dependent protein kinase 1
• Database Link:
  Entrez Gene 5170 Human
  UniProt ID O15530
• Background: PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
• Synonyms: 3-phosphoinositide-dependent protein kinase 1; hPDK1; PDK1; PDPK1
• Note: For research use only, not for use in diagnostic procedure.