GRLF1 (ARHGAP35) Rabbit Polyclonal Antibody

CAT#: TA392904

GRF-1 (phospho-Y1105) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.
• Specificity: p-GRF-1 (Y1105) polyclonal antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Tyr1105.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 172 kDa
• Gene Name: Rho GTPase activating protein 35
• Database Link:
  Entrez Gene 2909 Human
  UniProt ID Q9NRY4
• Background: GRF-1 (glucocorticoid receptor DNA-binding factor 1), also known as p190RhoGAP or simply p190, is a transcriptional regulator which binds to the promoter region of the glucocorticoid receptor gene and represses its expression. By repressing GR expression, GRF-1 acts to down-regulate Rho signaling, thereby mediating both actin cytoskeletal rearrangements and cell cycle events. Through its GAP domain, GRF-1 is thought to affect cytokinesis by regulating Rho activity; a regulation that is controlled by the ubiquination of the GTP binding region and subsequent degradation of GRF-1. Additionally, GRF-1 plays an important role in oligodendrocyte differentiation, a process that is absent in malignant glioma tumors, implicating GRF-1 as a possible tumor suppressor. GRF-1 expression is regulated by glucocorticoids and the expressed protein exists as two isoforms produced by alternative splicing events.
• Synonyms: ARHGAP35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; GRF1; GRLF1; KIAA1722; p190-A; Rho GAP p190A; Rho GTPase-activating protein 35
• Note: For research use only, not for use in diagnostic procedure.