Prominin 2 (PROM2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3D12]

CAT#: TA500349BM

Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI3D12 (formerly 3D12), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Positive lysates LY403405 (100ug) and LC403405 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of 35ug of cell extracts from human Liver carcinoma (HepG2) cells using anti-PROM2 antibody.

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  Western blot analysis of 35ug of cell extracts from human (HeLa) cells using anti-PROM2 antibody.

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  Western blot analysis of 35ug of cell extracts from human colon adenocarcinoma (HT29) cells using anti-PROM2 antibody.

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  Western blot analysis of 35ug of cell extracts from human Lung adenocarcinoma (A549) cells using anti-PROM2 antibody.

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  Western blot analysis of 35ug of cell extracts from canine Kidney (MDCK) cells using anti-PROM2 antibody.

• Immunofluorescent staining of Cos7 cells using anti-PROM2 mouse monoclonal antibody (TA500349).

• Immunofluorescent staining of A549 cells using anti-PROM2 mouse monoclonal antibody (TA500349).

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Specifications

Product Data

• Clone Name: OTI3D12
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human PROM2 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91.883 kDa
• Gene Name: prominin 2
• Database Link:
  NP_653308
  Entrez Gene 150696 Human
  UniProt ID Q8N271
• Synonyms: PROML2

Reference Data

• Protein Families: Transmembrane