PLK1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9E9]

CAT#: TA500388AM

PLK1 mouse monoclonal antibody, clone OTI9E9 (formerly 9E9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC201795 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody (TA500388), and then analyzed by flow cytometry (1:100).

Specifications

Product Data

• Clone Name: OTI9E9
• Applications: FC, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PLEK (NP_002655) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.1 kDa
• Gene Name: polo like kinase 1
• Database Link:
  NP_005021
  Entrez Gene 18817 MouseEntrez Gene 25515 RatEntrez Gene 5347 Human
  UniProt ID P53350
• Synonyms: PLK; STPK13

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation