GBP2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2G6]

CAT#: TA500655BM

Anti-GBP2 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (RC209939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2. Positive lysates LY401329 (100ug) and LC401329 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-GBP2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-GBP2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-GBP2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-GBP2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-GBP2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500655, Dilution 1:50)

• Anti-GBP2 mouse monoclonal antibody (TA500655) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2 (RC209939).

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Specifications

Product Data

• Clone Name: OTI2G6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human GBP2 expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.2 kDa
• Gene Name: guanylate binding protein 2
• Database Link:
  NP_004111
  Entrez Gene 2634 Human
  UniProt ID P32456
• Background: Interferons are cytokines that have antiviral effects and inhibit tumor cell proliferation. They induce a large number of genes in their target cells, including those coding for the guanylate-binding proteins (GBPs). GBPs are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). The protein encoded by this gene is a GTPase that converts GTP to GDP and GMP.
• Synonyms: DKFZp451C2311