ACOT12 Mouse Monoclonal Antibody [Clone ID: OTI1D3]

CAT#: TA502530S

ACOT12 mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (RC210445, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12. Positive lysates LY408930 (100ug) and LC408930 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC210445 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACOT12 antibody (TA502530), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody (TA502530), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-ACOT12 antibody (TA502530), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1D3
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACOT12 (NP_570123) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.9 kDa
• Gene Name: acyl-CoA thioesterase 12
• Database Link:
  NP_570123
  Entrez Gene 134526 Human
  UniProt ID Q8WYK0
• Synonyms: Cach; CACH-1; STARD15; THEAL

Reference Data

• Protein Pathways: Pyruvate metabolism