C1orf50 Mouse Monoclonal Antibody [Clone ID: OTI1B11]

CAT#: TA502912S

C1orf50 mouse monoclonal antibody, clone OTI1B11 (formerly 1B11)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502912)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C1orf50 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502912)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1orf50 (RC200134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1orf50. Positive lysates LY411368 (100ug) and LC411368 (20ug) can be purchased separately from OriGene.

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  Anti-C1orf50 mouse monoclonal antibody (TA502912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C1orf50 (RC200134 ).

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  Flow cytometric Analysis of Jurkat cells, using anti-C1orf50 antibody (TA502912), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-C1orf50 antibody (TA502912), (Red), compared to a nonspecific negative control antibody, (Blue).

• HEK293T cells transfected with either RC200134 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C1orf50 antibody (TA502912), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1B11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C1orf50 (NP_077002) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.7 kDa
• Gene Name: chromosome 1 open reading frame 50
• Database Link:
  NP_077002
  Entrez Gene 79078 Human
  UniProt ID Q9BV19
• Synonyms: MGC955