COG6 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B8]

CAT#: TA505418AM

COG6 mouse monoclonal antibody,clone 1B8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COG6 (RC207981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COG6. Positive lysates LY412375 (100ug) and LC412375 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-COG6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COG6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505418)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COG6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505418)

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  Anti-COG6 mouse monoclonal antibody (TA505418) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COG6 (RC207981).

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COG6 (NP_065802) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.1 kDa
• Gene Name: component of oligomeric golgi complex 6
• Database Link:
  NP_065802
  Entrez Gene 697092 MonkeyEntrez Gene 57511 Human
  UniProt ID Q9Y2V7
• Background: This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi apparatus. The encoded protein is organized with conserved oligomeric Golgi complex components 5, 7 and 8 into a sub-complex referred to as lobe B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
• Synonyms: CDG2L; COD2; SHNS