HDAC8 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B2]

CAT#: TA809667BM

HDAC8 mouse monoclonal antibody,clone OTI1B2, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC8 (RC208390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC8 (1:2000). Positive lysates LY413013 (100ug) and LC413013 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-243 of human HDAC8 (NP_060956) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.6 kDa
• Gene Name: histone deacetylase 8
• Database Link:
  NP_060956
  Entrez Gene 70315 MouseEntrez Gene 363481 RatEntrez Gene 55869 Human
  UniProt ID Q9BY41
• Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Synonyms: CDA07; CDLS5; HD8; HDACL1; MRXS6; RPD3; WTS

Reference Data

• Protein Families: Druggable Genome, Transcription Factors