RAD21 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2A12]

CAT#: TA811221BM

RAD21 mouse monoclonal antibody,clone OTI2A12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD21 (RC208262, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD21 (1:2000). Positive lysates LY401887 (100ug) and LC401887 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811221) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811221) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RAD21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811221) (1:2000)

Specifications

Product Data

• Clone Name: OTI2A12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 193-458 of human RAD21 (NP_006256) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.5 kDa
• Gene Name: RAD21 cohesin complex component
• Database Link:
  NP_006256
  Entrez Gene 19357 MouseEntrez Gene 314949 RatEntrez Gene 5885 Human
  UniProt ID O60216
• Synonyms: CDLS4; hHR21; HR21; HRAD21; MCD1; NXP1; SCC1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle