AFP ELISA Kit

CAT#: EA101028

AFP ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0 - 160 IU/mL
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 25 ul
• Specificity: Natural and recombinant human AFP
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of specimen sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti-AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of AFP in the specimen sample.