PAPP-A ELISA Kit

CAT#: EA101041

PAPP-A ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0.133 – 30 µg/mL
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human PAPP-A
• Sensitivity: 0.133 µg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The PAPP-A ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a polyclonal anti PAPP-A antibody. An aliquot of patient sample containing endogenous PAPP-A is incubated in the coated well with assay buffer. After incubation the unbound material is washed off. In the second incubation step a sandwich complex is formed with a polyclonal anti PAPP-A antibody peroxidase conjugate. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PAPP-A in the sample.