Mouse Chit1 ELISA KIT (1 x 96 wells)

CAT#: EA102710

For quantitative detection of mouse CHIT1 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Mouse CHIT1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 312pg/ml-20000pg/ml
• Specificity: This kit is used for quantitative detection of Mouse CHIT1
• Sensitivity: <50pg/ml
• Reactivities: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- mouse CHIT1 antibody | 1
  • Lyophilized recombinant mouse CHIT1 standard | 20ng/tube×2
  • Biotinylated anti- mouse CHIT1 antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1; 1 E4. It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract. CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form. Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease. The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme. Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
• Gene Symbol: Chit1
• Standard Curve:
  
  Mouse CHIT1 ELISA Kit Standard Curve