CHKL (CHKB) (NM_005198) Human 3' UTR Clone

CAT#: SC202954

3`UTR clone of choline kinase beta (CHKB) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHKB
• Synonyms: CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC
• ACCN: NM_005198
• Insert Size: 216 bp
• Sequence Data:
  Insert Sequence

  >SC202954 3'UTR clone of NM_005198
  The sequence shown below is from the reference sequence of NM_005198. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGTGTCCACTCCTCATCCTGACTCCACCCTCCCACTCCTTGGATTTCTCCTGGAGCCTCCAGGGCAGGAC
  CTTGGAGGGAGGAACAACGAGCAGAAGGCCCTGGCGACTGGGCTGAGCCCCCAAGTGAAACTGAGGTTCA
  GGAGACCGGCCTGTTCCTGAGTTTGAGTAGGTCCCCATGGCTGGCAGGCCAGAGCCCCGTGCTGTGTATG
  TAACAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005198.4
• Summary: 'Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]'
• Locus ID: 1120