MRPL4 (NM_146387) Human 3' UTR Clone
CAT#: SC203670
3`UTR clone of mitochondrial ribosomal protein L4 (MRPL4) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | MRPL4 |
Synonyms | CGI-28; L4mt |
ACCN | NM_146387 |
Insert Size | 221 |
Sequence Data |
>SC203670 3'UTR clone of NM_146387
The sequence shown below is from the reference sequence of NM_146387. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC CACCCCGTACCACTGTTGATGTGAAGCACCTCTTCTGAGCCAGGCCGAGCCCCTGGCCGACTTGGGAGCC TCAGGCCCACGCCCACCCTTCGAGGAAGGTGTCACCTGGACCCCTTCATTCCACGGAGGAAGCTGAGGCC ACAGGGAGCGGCCATCGCCATTGGGAAGGGGCGACTCCACGGAAAGCCCAGACGGGCTTCTGCATCCATT CCCTCTTTTTG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Reference Data | |
RefSeq | NM_146387.1 |
Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008] |
Locus ID | 51073 |
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