26S proteasome non ATPase regulatory subunit 12 (PSMD12) (NM_002816) Human 3' UTR Clone
CAT#: SC204794
3`UTR clone of proteasome (prosome macropain) 26S subunit non-ATPase 12 (PSMD12) transcript variant 1 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | PSMD12 |
Synonyms | p55; Rpn5; STISS |
ACCN | NM_002816 |
Insert Size | 364 bp |
Sequence Data |
>SC204794 3'UTR clone of NM_002816
The sequence shown below is from the reference sequence of NM_002816. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC CGCATCTCATAGCCAAAGAGGAGATGATACATAATCTACAATAAGGGTCTTAGTGCTTTAGAAAAAAGTT AAAATTGGAAGTCATTAAAAAAAGACTGTTATAATGGTGTATATGTTGGGGTTTTTTTTCTAAGCTTCTT TGTCTTAAATTTTAAAATAGTGAATATGTTTGAGACTCCCTTTGACCTTTCAGTTCCCCAAGTTCATTGT TAACTTTGCATTTGCAATTGGTGCAAAAATACAGATTTCTGTCGTCTGAATACACAAAAAGTTGTGTCAT AACTTACCCAGATATGTTTTTCTATCATTTGAAACCTTTTTAGCTACTGTTTGTTTTCATTCAACTAACA AACATATTCCAATA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_002816.3 |
Summary | 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]' |
Locus ID | 5718 |
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