Glutathione Reductase (GSR) (NM_000637) Human 3' UTR Clone

CAT#: SC207968

3`UTR clone of glutathione reductase (GSR) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GSR
• Synonyms: GR; GSRD; HEL-75; HEL-S-122m
• ACCN: NM_000637
• Insert Size: 606 bp
• Sequence Data:
  Insert Sequence

  >SC207968 3'UTR clone of NM_000637
  The sequence shown below is from the reference sequence of NM_000637. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAGAAGAGCTGGTCACACTTCGTTGAGAACCAGGAGACACGTGTGGCGGGCAGTGGGACCCATAGATCT
  TCTGAAATGAAACAAATAATCACATTGACTTACTGTTTGAGTTTTATGTATTTCTTTATTTTAATCAGGA
  TCTTCTGATAGTGGAAATTTTTAGTACATAATAGAACTTATTTATGGAGTTAGAAATTTGTAGTGTTATC
  CAGGATTGATTTTCATTTGATCACATCTCACAGTAATTAATATTTTCAAGTTTTTTTTTTATTAACAGCT
  CTGTGCTAGTTTTTTTTTTCTGTTTTAGCCTCATCCCAAATATAAAGCTTTGTGAAGTACAATTAACTTA
  ATGTACTTGAATGAATAGAACTTGCTACTTTTTTTTTTTTTTTTTTTGAGACAGAGTTTTGCTCTCATTG
  CCCAGGCTGGAGTGCGGTGGTGCTATTTCAGCTCACCACAACCTCTGCCTCCTGGGTTCAAGTGATTCTC
  CTGCCTTAGCCTCCCGAATAGCTGGAATTACAGGCACGCACCACCATGCCTGACTAATTTTGTATTTTTA
  GTAGACATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTCAAACTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000637.2
• Summary: 'This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]'
• Locus ID: 2936