GCAP1 (GUCA1A) (NM_000409) Human 3' UTR Clone

CAT#: SC208665

3`UTR clone of guanylate cyclase activator 1A (retina) (GUCA1A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LOC118142757
• Synonyms: GUCA1A; GUCA1ANB-GUCA1A
• ACCN: NM_000409
• Insert Size: 663 bp
• Sequence Data:
  Insert Sequence

  >SC208665 3'UTR clone of NM_000409
  The sequence shown below is from the reference sequence of NM_000409. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAGGGGGCTGACGAGGCCGCTGAGGCAGCCGGCTGAGTGCACCGCCCGGCTGCTTCTGCACTAGCGGG
  TGGGGTGGTATGGTGGTGCCTGTTGGTGGTGTTCTTGTCTTAACCCTAGATAGAATCTAATGAACTCAGA
  GGCTTAGCTCGCCTCTTTAGGGTCCATGGTGGCAGCAGAGAGGCAGAAGTGGGAGTCCAGAGCCAGGAAC
  AGTGAAGGATGGTTCCTGGCCCCTCTGAGTGACAGCTGGTGGCAGCACTCCTTGCTGGGGGGCACTGTTC
  AACATCCCTCTGCCGTCGGGTGACCCCCTAGCCCTTCTGACTCCTCTCCCAGCTTTTCCCAGCTTTCCCC
  ACTGAGCTTCTCCAGTCCATGCTCTTCTGGACGTGGACTCTCTGAGGCAGAACTGAGCTTTTCCAGGCCT
  CTTATGGAATCCTGCAGATCCAGTGGCTGCAGCTTCAATCCCAGTGCTGCAATCACACATCCATTCTGCC
  CTGGGGGACCCTGGAGCCTACTTGTGCGCTTTGCATTTCATTGATTGACGCCTCCCTTCAACAAGCATTT
  ACTGAGCGCCTACTATGTACTAATGCTAGATGTTAGATGTACAAAGAAGACAGTTTTCATCCTCTAGGAA
  CTCATAGGCTAATGGTGAGACACACAGACAAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000409.3
• Summary: 'This gene encodes an enzyme that plays a role in the recovery of retinal photoreceptors from photobleaching. This enzyme promotes the activity of retinal guanylyl cyclase-1 (GC1) at low calcium concentrations and inhibits GC1 at high calcium concentrations. Mutations in this gene can cause cone dystrophy 3 and code-rod dystrophy 14. provided by RefSeq, Jul 2020]'
• Locus ID: 2978