PIF1 (NM_025049) Human 3' UTR Clone

CAT#: SC208666

3`UTR clone of PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PIF1
• Synonyms: C15orf20; PIF
• ACCN: NM_025049
• Insert Size: 677
• Sequence Data:
  Insert Sequence

  >SC208666 3'UTR clone of NM_025049
  The sequence shown below is from the reference sequence of NM_025049. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCCTCAGACCAGGAGAACATGGACCCAATCCTCTGAGCCTCACCCACAAAGAGAAGACAAAGGGTGGCC
  TGTGGCCTCCCCGTCCTCCTGCTCCCTAGTGGCCCAGGGCCCCAGGGAATAACTGGAGTAGGCAGGCAGT
  GTCCCCTTCTGTATTTTTTAGGGACTCTCAGCCTCCTGGCAGGGTTAAAGGGAGAGTACTTTAAACCCAT
  ATACCAGCTGTGCTTCAGTTTCTCTCACTTTGCCCTGGGTAAGCTGCTGTAGGGTCAGAAGTAACCCTTT
  CTGTGCCAGTTGAGAATGAGCCTGTGTGGTAGCTGATGTCAGAGGACAAAGCTCTCTGCAAGGGCTGGAC
  ACAGAGCTGCAGAGTCCTGAACATCCCTCCTTTCAGGCTGCAGAAGGGAGAGGCAATGAAGACAGGTGCT
  CCGGAAGCAGCATCAGGGCTCTTGGAGGGGACTGGTGGGGACTCAGGCTGGGTGCAGCCTCCAAACAGAG
  AACGGAACTTAGGTGTGTCTCTACAGCTAGGCCCAGCCTAGCCCAGCCCAGAACAAACACCCTTCAGAGC
  CTAACCAAAGAACATAAGCTGCAAAATGTGCACCCATATTTTAAGCTGCTTTTTCAGGGGATAAATAGTG
  TTTGGGGACATTGAAATGGATGTTCTCAGGTTGTATTTATTTCGGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_025049.2
• Summary: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]
• Locus ID: 80119