RNase H1 (RNASEH1) (NM_002936) Human 3' UTR Clone

CAT#: SC209018

3`UTR clone of ribonuclease H1 (RNASEH1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RNASEH1
• Synonyms: H1RNA; PEOB2; RNH1
• ACCN: NM_002936
• Insert Size: 715
• Sequence Data:
  Insert Sequence

  >SC209018 3'UTR clone of NM_002936
  The sequence shown below is from the reference sequence of NM_002936. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GATTAGCCAGAGAAGGAGCTAAACAATCGGAAGACTGAGCCATGTGACTTTAGTCCTTGGGAGAACTTGA
  GCCAGCGGCTGTCTTGCTGCCTGTACTTACTGGTGTGGAAAATAGCCTGCAGGTAGGACCATTGCAGTGA
  TGGGCAGATGCGTCTTTCACACGGAATCAGGCACAGTGGCCTTCTGTGACATGTGTTTATAAAAAATGGT
  TAAGTATATAATAAATTGAACATCTTTGAGATTGGAGAATTATGTGAGATTTCCACATTATGTTTACTGG
  GTTCAATACTGTCCTTGCTTGTTTTATTGCAGGCAAGCAAGGCAAATGGCCTAAAATGCTGTGGCTTATA
  TTTTGATAAGAAATCAAAAAACCATTGGTTAAAAGATGCAACTCAGAAGTCTGGAAGTATTCTGAAAGCA
  TCCATTTACCGTCCAGTTGACAGGTTTGAGTCTCCTGCTTGTATAGGTGACTTGTGCCCATGGGTACATT
  AAAGGAACATGCTGCCCAGGGCCTGGGCGGACAGCTCAGTGGGCAGGATGTGTGCTGGGTCTCAGCCCCA
  TGTGCCTGCTTGCTGGGCAGTTAGTATAGGGCAAAGCCTGCCTGCGGCGACCCTGGCTGCTAGGCCATTC
  TCTAGGAACAGCTGCGACTCATAAAGACCAAGAAGCATAAATAAACTTTCAAAAATTTATTTGGCTCTTT
  CGTTAAAAACTGTGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_002936.3
• Summary: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]
• Locus ID: 246243