Nck (NCK1) (NM_006153) Human 3' UTR Clone

CAT#: SC209022

3`UTR clone of NCK adaptor protein 1 (NCK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NCK1
• Synonyms: NCK; nck-1; NCKalpha
• ACCN: NM_006153
• Insert Size: 682 bp
• Sequence Data:
  Insert Sequence

  >SC209022 3'UTR clone of NM_006153
  The sequence shown below is from the reference sequence of NM_006153. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCTTGTCAAGCATTTATCATGATACTGCTGACCAGAAGTGACTGCTGTGTAGCTGTAATTTGTCATGTA
  ATTGAAGACTGAGAAAATGTTGGGTCCAGTCGTGCTTGATTGGAAATTGTTGTTTCTAAATCTATATGAG
  AATTGACAATAAGTATTTTTATTATAACTCAGCCCATACATATATACTATGTATGCAGTGCATCTGCATA
  GAACAGTTCCTTATCCTTGGCCTTCTGTTTTATTGTTTTTTTCTTTGCTGTTTTCCCTTTGCTTCTAATA
  TTACAGTTTTGTATTTTGTAAACAAAAATCAAATAATGCATATCAGAATCTTTATATGGAAGAAATCCTT
  TATTGCCTTTCCTTTGTTTCCTTGTAAAGGCACCCTGTTCTGTTATGGTTTTTCATTATATAAAATTATT
  ATATCTATATATGACATATGCTAAAATTTCTTGGAGAGTGTTAATCTTTTCTGTGACTAAATAGCAATAA
  TAAGTGGAAAATTAGAAATTATTTCCAGGTATTATATTTGTCACAGGCCATTGTAAATACCAAGTATATT
  GTGTCTGCCATAATTTTTAAAAATACATTCATTGTCTTCAGTCATACAGCAAGACACATGAGACATAGAT
  TAGAAAACATGTTGTACAATTTTAATTTACAACTGTTGGAAATAAAAATCAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006153.3
• Summary: 'The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]'
• Locus ID: 4690