PKR (EIF2AK2) (NM_001135651) Human 3' UTR Clone

CAT#: SC209230

3`UTR clone of eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EIF2AK2
• Synonyms: EIF2AK1; PKR; PPP1R83; PRKR
• ACCN: NM_001135651
• Insert Size: 735 bp
• Sequence Data:
  Insert Sequence

  >SC209230 3'UTR clone of NM_001135651
  The sequence shown below is from the reference sequence of NM_001135651. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGTGGAAGAAAAGCCCAGAGAAAAATGAACGACACACATGTTAGAGCCCTTCTGAAAAAGTATCCTGCT
  TCTGATATGCAGTTTTCCTTAAATTATCTAAAATCTGCTAGGGAATATCAATAGATATTTACCTTTTATT
  TTAATGTTTCCTTTAATTTTTTACTATTTTTACTAATCTTTCTGCAGAAACAGAAAGGTTTTCTTCTTTT
  TGCTTCAAAAACATTCTTACATTTTACTTTTTCCTGGCTCATCTCTTTATTCTTTTTTTTTTTTTAAAGA
  CAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAATGACACAGTCTTGGCTCACTGCAACTTCTGCCTCT
  TGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGATTACAGGCATGTGCCACCCACCCAAC
  TAATTTTTGTGTTTTTAATAAAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTC
  AAGTAATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGGATGAGCCACCGCGCCCAGCCTCAT
  CTCTTTGTTCTAAAGATGGAAAAACCACCCCCAAATTTTCTTTTTATACTATTAATGAATCAATCAATTC
  ATATCTATTTATTAAATTTCTACCGCTTTTAGGCCAAAAAAATGTAAGATCGTTCTCTGCCTCACATAGC
  TTACAAGCCAGCTGGAGAAATATGGTACTCATTAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001135651.1
• Summary: 'The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
• Locus ID: 5610