DNA Primase (PRIM2) (NM_000947) Human 3' UTR Clone

CAT#: SC209384

3`UTR clone of primase DNA polypeptide 2 (58kDa) (PRIM2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRIM2
• Synonyms: p58; PRIM2A
• ACCN: NM_000947
• Insert Size: 710 bp
• Sequence Data:
  Insert Sequence

  >SC209384 3'UTR clone of NM_000947
  The sequence shown below is from the reference sequence of NM_000947. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATATGGAAGGACTAGAAGATTACTTTAGTGAAGATTCTTAGGCAGTTTTATAACCCTTTTTCCTCAAT
  AGCCTGTTTCCTGTTTTTAAGATTTTGCCTTTGTTGTTGAAAAAGGGTTTCACTCTGTCACCAAGGCTTA
  GTGCAGTGACACAATTACAGCTGATTGCAGCCTTGACCTTCCCAGCTCAAGTGATCCTCCTACCTCAGCC
  TCCCAAGTAGTTAGGACCACAGGTGTGCACCTCATATCCAGATAATTTTTTTCAATTTTTTTTTGTAGAG
  GTGGGGGGTCTCCCTATGTTGCCCAGGCAGATCTCAGACTCCTGGGCTCAAGCGATCCTCACACCTCAGC
  GTCCCAGAGTGCTGGGATTACGGTTGTGAGCCACTGTGCCTGGCCTTTTTTTTTTTTTTTAACCTTTTTG
  TTTAACTTCTCTCTTCACTGCATCCCAATCCATCTACAGGCATGCACACTTATTAGGAAAGGAGGTTTGA
  GGTAACAACAGAGACTTTCACTATATTTTGCTTTGACAGAAGGAAAGAGGAGGAGTTTCTATTAAAATCT
  GTCACTTGAGTGATGTCATTTAAGTCCTATTTTAGGAGATAAAAACAGCTTTGGGGACTGGTTAAAGTCC
  CCCAGAAACTACAATAAAGAACAACTTTTGTTTTAACTCTTAATCACTTTGTAATTTTGACTCAATCCTT
  TTCTGGACCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000947.2
• Summary: 'This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]'
• Locus ID: 5558