HARS2 (NM_012208) Human 3' UTR Clone

CAT#: SC209731

3`UTR clone of histidyl-tRNA synthetase 2 mitochondrial (putative) (HARS2) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HARS2
• Synonyms: HARSL; HARSR; HisRS; HO3; PRLTS2
• ACCN: NM_012208
• Insert Size: 747
• Sequence Data:
  Insert Sequence

  >SC209731 3'UTR clone of NM_012208
  The sequence shown below is from the reference sequence of NM_012208. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAAGCGACTGTCTGAGTCTTGATCCTTGCCTGATTCCCATCTGCTGCTCTTTGTAGAAAAGGTTTCCTC
  TAGAACTGAATTCCTCTGGAATTGAGTGATGGACTTCACAACAACTAGCCAGGAAGGGTGACAAGTACCT
  TCTGCCTCCTCCATTCTTCCTGGGTGCAGAACTGTAGAAGACCCTGAGGACTCCTGGGCTAGTGTGAGCA
  GCTATTCTGCATGGGTGCAGATGGCTGGATGTGAAAGAGACATGCTCTAGCTGCAGAGGCAAATTTGAAG
  TGCCACGGAACGTTGTCAAGAGGTAGTGAGATTGTTGCTGTGAGCAAGGCTCTGGGAGAGTCACCTCAGG
  CTCAGGATTCTGAACCATTGAGATCAGAAACCAGATACTTAGCCTTCTACTGTAGCTACGGAACCAGATT
  CTGGTGAATTTGTCCACAATCAGCCATTGGCCTGTCTGGGGAGTTTTTGGAAGACAGCAAAGAGGGGCAA
  TGGCTGCTTTCAGCTTTGAGGACAGATGCTATCCTAAGGGCATGGCAGTACCCATGTTGATTTGACATCT
  CTCTAGCCCATCCATTGCTTACAGTAGAAGAGTGGGGCTGTATGCTTGATAATCTTGAACATTGAACTTA
  ATAGATGACTTGACAAGGTAGAGATCTGATATTTTGAGATTTTGGAGACCATTTCCTGTGCCAGAATCAC
  TGCTCTATTCCATACCGTGCCATGGAGGCTGTTTTAGAAGTGGTTGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_012208.2
• Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
• Locus ID: 23438