LATS1 (NM_004690) Human 3' UTR Clone

CAT#: SC210118

3`UTR clone of LATS large tumor suppressor homolog 1 (Drosophila) (LATS1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LATS1
• Synonyms: WARTS; wts
• ACCN: NM_004690
• Insert Size: 842
• Sequence Data:
  Insert Sequence

  >SC210118 3'UTR clone of NM_004690
  The sequence shown below is from the reference sequence of NM_004690. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAAACACAGGCTCAGAGATTAAAAATCGCGATCTAGTATATGTTTAACACACTAGTAAATAAATGTAATG
  AGGATTTGTAAAAGGGCCTGAAATGCGAGGTGTTTTGAGGTTCTGAGAGTAAAATTATGCAAATATGACA
  GAGCTATATATGTGTGCTCTGTGTACAATATTTTATTTTCCTAAATTATGGGAAATCCTTTTAAAATGTT
  AATTTATTCCAGCCGTTTAAATCAGTATTTAGAAAAAAATTGTTATAAGGAAAGTAAATTATGAACTGAA
  TATTATAGTCAGTTCTTGGTACTTAAAGTACTTAAAATAAGTAGTGCTTTGTTTAAAAGGAGAAACCTGG
  TATCTATTTGTATATATGCTAAATAATTTTAAAATACAAGAGTTTTTGAAATTTTTTTGAAAGACAGTTT
  TAGTTTTATCTTGCTTTAACCAAATATGAAACATACCCCCTATTTTACAGAGCTCTTTTTTCCCCTCATA
  ACCTTGTTTTTGGTAGAAAATAAGCTAGAGAAATTAAGCCATCGTGTTGGTGAGTGTTCCTAGGCTAATG
  ATAATCTGTATAATTCACATCCTGAAACTAAGGAATACAGGGTTGAAAAAATATTAATATGTTTGTCAGA
  AGGAAAAATAATGCATTTATTTTCCCCCCCACCCCCCGCCCCATGGAATATTTAATCTATTTAATCTTCT
  TGCATTTATTTCTCAAGAATTACTGGCTTTAAAAGAAGCCAAAGCACTACTAGCTTTTTTTCCATATTGG
  TATTTTTGATGCTGCTTCCAATTTTAAAAGGGAACAAAGCTGCCATAAATCGAAATGTTCAATACTAAAA
  GC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004690.2
• Summary: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]
• Locus ID: 9113