CHMP4B (NM_176812) Human 3' UTR Clone

CAT#: SC210197

3`UTR clone of chromatin modifying protein 4B (CHMP4B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHMP4B
• Synonyms: C20orf178; CHMP4A; CTPP3; CTRCT31; dJ553F4.4; Shax1; SNF7; SNF7-2; Vps32-2; VPS32B
• ACCN: NM_176812
• Insert Size: 824
• Sequence Data:
  Insert Sequence

  >SC210197 3'UTR clone of NM_176812
  The sequence shown below is from the reference sequence of NM_176812. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAATTGGAGAACTGGGCTGGATCCATGTAATGGGGTCCAGCGCTGGCTGGGCCCAGACAGACTGTGGTG
  GCCTGCGCAGCGAGCAGGCGTGTGCGTGTGTGGGGCAGGCAGGATGTGGTGCAGGCAGGTTCCATCGCTT
  TCGACTCTCACTCCAAAGCAGTAGGGCCGCGTTGCTGCTCACTCTCTGCATAGCATGGTCTGCACCTGGG
  AGATGGGCGGGGGGAGGGGGGCGGGCGGGGTGGGAAGTGCCTGCTGTTTATAATGTTGAATTTCTGTAAA
  ATAAACTGTATTTGCAAATCCAACATTGAGCTTCTGGACTACGCTGACTCCACTGCTGAATCCTCAATGG
  AAAGGGTCGACTGGTTGCAGTTGAAATGACCTGAAATGTAGCCTCTGTCCTTGTAAGTCAGTTGACTTGC
  CGCACATCTCTTTGTGTACTTGTACGGTACTGGCAGAAAAGTCATTTTTCAAAAGCCATAGGCTTTTCCT
  TGCCCTTAGCTGTAATAATGCATCTGATTTTGATTTCCTCCAGAGCTGTGTTTCTGTCCATCACCTGTGT
  ATTGGCCCTGTGTTTACCACTCTGGCCCACTCCTCACCCCCTTGCTCCCCTGGTCTTCTGGAGTTTGTGA
  CATTGATTTGAAATGGATGGTGTTCTCTTGAGAGCAAGTGAGATTGTTAGAATTAAGTTCCAACTATACA
  GTTTTCTAACATAGCTATAAGGTCCTTGTTGCTGTTTGTGATAACTGATAGATAACTCATTGGAAACGTG
  CATACATTTATATTCAGATGAAATTATGGTTTGCACTGTCTATTAAATATCTCG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_176812.4
• Summary: This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts. [provided by RefSeq, Oct 2009]
• Locus ID: 128866