LEFTY2 (NM_003240) Human 3' UTR Clone

CAT#: SC210384

3`UTR clone of left-right determination factor 2 (LEFTY2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LEFTY2
• Synonyms: EBAF; LEFTA; LEFTYA; TGFB4
• ACCN: NM_003240
• Insert Size: 839 bp
• Sequence Data:
  Insert Sequence

  >SC210384 3'UTR clone of NM_003240
  The sequence shown below is from the reference sequence of NM_003240. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAAGGAGGCTCCAGCCATAGGCGCCTGGTGTATCCATTGAGCCCTCTAACTGAACGTGTGCATAGAGGTG
  GTCTTAATGTAGGTCTTAACTTTATACTTAGCAAGTTACTCCATCCCAATTTAGTGCTCCTGTGTGACCT
  TCGCCCTGTGTCCTTCCATTTCCTGTCTTTCCCGTCCATCACCCATCCTAAGCACTTACGTGAGTAAATA
  ATGCAGCTCAGATGCTGAGCTCTAGTAGGAAATGCTGGCATGCTGATTACAAGATACAGCTGAGCAATGC
  ACACATTTTCAGCTGGGAGTTTCTGTTCTCTGGCAAATTCTTCACTGAGTCTGGAACAATAATACCCTAT
  GATTAGAACTGGGGAAACAGAACTGAATTGCTGTGTTATATGAGGAATTAAAACCTTCAAATCTCTATTT
  CCCCCAAATACTGACCCATTCTGGACTTTTGTAAACATACCTAGGCCCCTGTTCCCCTGAGAGGGTGCTA
  AGAGGAAGGATGAAGGGCTTCAGGCTGGGGGCAGTGGACAGGGAATTGGGATACCTGGATTCTGGTTCTG
  ACAGGGCCACAAGCTAGGATCTCTAACAAACGCAGAAGGCTTTGGCTCGTCATTTCCTCTTAAAAAGGAG
  GAGCTGGGCTTCAGCTCTAAGAACTTCATTGCCCTGGGGATCAGACAGCCCCTACCTACCCCTGCCCACT
  CCTCTGGAGACTGAGCCTTGCCCGTGCATATTTAGGTCATTTCCCACACTGTCTTAGAGAACTTGTCACC
  AGAAACCACATGTATTTGCATGTTTTTTGTTAATTTAGCTAAAGCAATTGAATGTAGATACTCAGAAGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003240.3
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. This gene is closely linked to both a related family member and a related pseudogene. This gene encodes multiple isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]'
• Locus ID: 7044