PYK2 (PTK2B) (NM_173176) Human 3' UTR Clone

CAT#: SC210721

3`UTR clone of PTK2B protein tyrosine kinase 2 beta (PTK2B) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTK2B
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• ACCN: NM_173176
• Insert Size: 897
• Sequence Data:
  Insert Sequence

  >SC210721 3'UTR clone of NM_173176
  The sequence shown below is from the reference sequence of NM_173176. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAAGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGCGTCT
  TCCGCCCCTGCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCCA
  AGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCACCCCTACCCCTGGCTGTACTGCTC
  AGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAGG
  GGGACTGCTGCTGCCTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGG
  AGGTGTCACATGGTGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTT
  TCCCTTCCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTGGGGG
  TCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGAAATTTATTTAATGT
  GAGTTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTA
  ATTGGGAGTCCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCAGGGGGAAGAAGCAGAGAGATG
  CGGCCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTC
  CCTTGCTTTTCCCTCTTTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCT
  TTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGGTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_173176.1
• Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 2185