CHRNB1 (NM_000747) Human 3' UTR Clone

CAT#: SC210838

3`UTR clone of cholinergic receptor nicotinic beta 1 (muscle) (CHRNB1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHRNB1
• Synonyms: ACHRB; CHRNB; CMS1D; CMS2A; CMS2C; SCCMS
• ACCN: NM_000747
• Insert Size: 933 bp
• Sequence Data:
  Insert Sequence

  >SC210838 3'UTR clone of NM_000747
  The sequence shown below is from the reference sequence of NM_000747. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGACGCCACGTACCACTTGCCCCCTCCAGACCCCTTTCCTTGAAGACTGGAGGGTTGAGACCCAGGCCC
  CCTGCCAGTTGAAGTGAGAGTTTGGTGATACTGTCAAGCCCTATCCTTCTCTGCCTCTTAACTCCTTCAC
  GAGGAATCTGGGCCTCTTATTTCGTTTCTGGGGACTGCATTGGACTGAGGGCTGGGTAGGCAGGTGTCTT
  GGACCCACCTGAAATGCAGTATCATCTGATTTACTCTTTGGGATCTTGAAGAAGCTCTTTTGGGTATCAA
  CACCTAGGTCGCCAGTGAAATAGAACACAGAACAGGAACTAGATTATAAGCCTTATGAGGTCAAGAAATG
  TGACTTGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCAC
  CTGAGGTCGGGAGTTTGAGACCAGCCCGACCAACATGGAGAAACCCTGTCTCTACTAAAAATACAAAATT
  AGCCAGGTGTGGTGGTACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGAGAATCACTCGAAC
  CCGGGAGGCAGAGGTTGCAGTGAGTCAAGATCACGCCATTGCACTCCAGCCTGGGCAACAAGAGCGAAAC
  TCCATCTCAAAAAAAAAAAAGTGTCTTGTTCACTGCAGCATGCCCAGTGCCACGCACAGGTGCTGACCTA
  TAGTAAGTGCTTAACATTTGTTGAATAGGGGAAAGAAATTTCCGGAAGTAAATACAGCAATTAATAATGT
  TTATAAGCTGGGCATGGTCGCTCAGGCCAGTAATCCCAGTGACTTAGGAGGCTGAGGTGGGAGGATTACT
  TGAAACCCACAGTTTGAGACCAGCCTGGGCAACATAGTGAGACCCTGTCTCTAAAAAAATAAAAATAGAA
  ATAAAGTAGTGCTTATTGTTTGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000747.2
• Summary: 'The muscle acetylcholine receptor is composed of five subunits: two alpha subunits and one beta, one gamma, and one delta subunit. This gene encodes the beta subunit of the acetylcholine receptor. The acetylcholine receptor changes conformation upon acetylcholine binding leading to the opening of an ion-conducting channel across the plasma membrane. Mutations in this gene are associated with slow-channel congenital myasthenic syndrome. [provided by RefSeq, Jul 2008]'
• Locus ID: 1140