CYP1A1 (NM_000499) Human 3' UTR Clone

CAT#: SC211305

3`UTR clone of cytochrome P450 family 1 subfamily A polypeptide 1 (CYP1A1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP1A1
• Synonyms: AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX
• ACCN: NM_000499
• Insert Size: 946 bp
• Sequence Data:
  Insert Sequence

  >SC211305 3'UTR clone of NM_000499
  The sequence shown below is from the reference sequence of NM_000499. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCACTTCCAAATGCAGCTGCGCTCTTAGGTGCTTGAGAGCCCTGAGGCCTAGACTCTGTCTACCTGGTCT
  GGTTGGGCAGCCAGACCAGCAGGCTGGCCTATGTGGTCTAAGGTTCAGCCTGAAACTCATAGACACTGAT
  CTGGCTGCAGTTTTGCTATCTGGGCTGTGGGCAAGCCTAAGGGATCCTGCCTGCCCCTACCCTGGACTTG
  CCTCTGCACACCCTCCAGAGACAACAGGTAAAACAGGGCCACATAGATGCTGATGGAGCCTTCCCAAGTT
  GTGCTTGAGCCAGGAGGCCTGCTAGGGTTAGGAGGTCCTTAGGCCTCTGAGAAGCTCTGAAGAACTCTCT
  GGAAGCCCCTGGGCCCAGTACCTAGCTGGCTCTGTGAGGGTGCTGACTGGCTTCAGCAAGTTAGAACTAG
  CCAAACCAGGACCCTGTCCAATCTTTGACAATTGGGAGCTGCCAAGAGTGAAGGGAAGAGACAGCCCAGG
  ATACTGGCACAGAGGTAGTCTCACTGCTTGAACTAGGCTGAGCAATCTGACCCTATGGGTCTAGGACACA
  GTTCCTGGGAACATCACATTCCTCTGCCCTTCCTGCAGGCAGGAACAAACAGGGCTGCCTTCTGGCCTTG
  TAAGACCCTTATTGCTGTCCTGGAGGGGCTGGGGACTTGTGTCTGCGGGGATCAGAGCGCACAGGGAGTG
  CACATATCCAGGCACCAGGACTAGGGCTGGAGTGAGGGGGGGGTATTTCAATTACCTTCTATTGGTCTCC
  CTTCTCTACACTCTTGTAATAAAATGTCTATTTTTAATGTTTGTACACAACAATCCTTCTATTCTAGCCT
  GCATTGAGCTTGCATGCTTGCATAAGAGCTTAAGAACCATTGATTTAATGTAATAGGGAAAATTCTAACC
  CAGGTATCCAAAAATGTGTAAGAACAACTACCTGAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000499.3
• Summary: 'This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]'
• Locus ID: 1543