p107 (RBL1) (NM_002895) Human 3' UTR Clone

CAT#: SC211565

3`UTR clone of retinoblastoma-like 1 (p107) (RBL1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RBL1
• Synonyms: CP107; p107; PRB1
• ACCN: NM_002895
• Insert Size: 1003 bp
• Sequence Data:
  Insert Sequence

  >SC211565 3'UTR clone of NM_002895
  The sequence shown below is from the reference sequence of NM_002895. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGACTACAGGATGTTGTCAGTGAAAGAGCAAATCATTAATGTTGTTCTTGTTTCTATGATAAAAGCACTT
  TCAGATTGTTCTGCAGAAAGTTGGAGCTCTGTCCTTCAAACCTTTTAGCCCTATAGATGATAAATATCAC
  TGGGTTATAAGAAAAAATTGCACAAAAATTATGTGCTTTTTAAAATATTTATCCAAAATGTAGTTGACAG
  AGATGTATTTTGAGTTGGATTGGAAAGGAATATTTTAAGTGCCTTTTAAAAATACTAATAGTCCGGCCAG
  GCGCTGTGGCTCACGCCTGTAATCCCAGGACTTTGGGAGGCCAAGGCGGGCAGATCACCGGAGGTCAGGA
  GTTCGAGACCAGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAATACAAAATTAGCCGGGTGTGG
  TGGCGCATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGAATTGCTTGAACCCAGGAAGCGGAGGT
  TGTGGTGAGCCAAGGTTGCGCCACTGCACTCCAGCCTGGGCAACAAGAGTAAAACTCCATCTCAAAAAAT
  ATATATATATATATAAATAGGGAATTTTTTTTAATGTTTGCTCCTTGAGTTTTCAAGATGAAATAAGGAG
  AAACCCCATAACTTTTTAGCTCTCTTTTAAAAATAAATGTCTCCTTCTGTGTTCTGTAATATGAGGATAA
  ATAATCTACTTTTGATAGCATGCTTTGAGATATTTGTATTCTTAATTTAATATTGAAGGAAGGGGTTGGT
  TCCCATAGTACCTGGCCAGAGGGTTATATACCATCCTGTCTCTGGCCCACTGTGGTAATTCCACATCCAG
  GTACCACCGCCATTCAGAGTTGTCTCACCCCTTCTCGTGCCTTTTCTCTCCTTGAGCTTTATAAACAGAT
  TTGTACCTGAGCCCCAGTGCTACACTCATCCTTGCTTTCCAGCCCTAAGCCATCTCAGCCTGATGTCACA
  ACCCAATAAATGGGGCACTTTCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002895.2
• Summary: 'The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 5933