Acetylcholinesterase (ACHE) (NM_015831) Human 3' UTR Clone

CAT#: SC211570

3`UTR clone of acetylcholinesterase (Yt blood group) (ACHE) transcript variant E4-E5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACHE
• Synonyms: ACEE; ARACHE; N-ACHE; YT
• ACCN: NM_015831
• Insert Size: 997 bp
• Sequence Data:
  Insert Sequence

  >SC211570 3'UTR clone of NM_015831
  The sequence shown below is from the reference sequence of NM_015831. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACCAGCTTCTCCTCCTCTTCCTCTCCCACCTCCGGCGGCTGTGAACACGGCCTCTTCCCCTACGGCCACA
  GGGGCCCCTCCTCTAATGAGTGGTCGGACCGTGGGGAAGGGCCCCACTCAGGGATCTCAGACCTAGTGCT
  CCCTTCCTCCTCAAACCGAGAGACTCACACTGGACAGGGCAGGAGGAGGGGGCCGTGCCTCCCACCCTTC
  TCAGGGACCCCCACGCCTTTGTTGTTTGAATGGAAATGGAAAAGCCAGTATTCTTTTATAAAATTATCTT
  TTGGAACCTGAGCCTGACATTGGGGGGAAGTGGGAGGCCCCGGACGGGGTAGCACCCCCCATTGGGGCTA
  TAACGGTCAACCATTTCTGTCTCTTCTTTTTCCCCCAACCTCCCCCTCCTGTCCCCTCTGTTCCCGTCTT
  CCGGTCATTCTTTTCTCCTCCTCTCTCCTTCCTGCTGTCCTTCTCCGGCCCCGCCTCTGCCCTCATCCTC
  CCTCTCGTCTTTCGCACATTCTCCTGATCCTCTTGCCACCGTCCCACGTGGTCGCCTGCATTTCTCCGTG
  CGTCCTCCCTGCACTCATACCCCCCCTTCAACCCGCCCAAATGTCCGATCCCCGACCTTCCTCGTGCCGT
  CCTCCCCTCCCGCCTCGCTGGGCGCCCTGGCCGCAGACACGCTCGACGAGGCGGAGCGCCAGTGGAAGGC
  CGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTACAGCAAGCAGGAT
  CGCTGCTCAGACCTGTGACCCCGGCGGGACCCCCATGTCCTCCGCTCCGCCCGGCCCCCTAGCTGTATAT
  ACTATTTATTTCAGGGCTGGGCTATAACACAGACGAGCCCCAGACTCTGCCCATCCCCACCCCACCCCGA
  CGTCCCCCGGGGCTCCCGGTCCTCTGCATGTCTCAGGCTGAGCTCCCTCCCCCGCGGTGCCTTCGCCCCT
  CTGGGCTGCCAATAAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_015831.2
• Summary: 'Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]'
• Locus ID: 43