GABA A Receptor alpha 5 (GABRA5) (NM_001165037) Human 3' UTR Clone

CAT#: SC211608

3`UTR clone of gamma-aminobutyric acid (GABA) A receptor alpha 5 (GABRA5) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GABRA5
• Synonyms: MGC138184
• ACCN: NM_001165037
• Insert Size: 1008 bp
• Sequence Data:
  Insert Sequence

  >SC211608 3'UTR clone of NM_001165037
  The sequence shown below is from the reference sequence of NM_001165037. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGGTGATAAAAGGAGCCGCCTCTCCAAAATAACCGGCCACACTCCCAAACTCCAAGACAGCCATACTTCC
  AGCGAAATGGTACCAAGGAGAGGTCTTGCTCACAGGGACTCTCCATATGTGAGCACTATCTTTCAGGAAA
  TTTTTGCATGTTTAATAATATGTACAAATAATATTGCCTTGATGTTTCTATATGTAACTTCAGATGTTTC
  CAAGATGTCCCATTGATAATTCGAGCAAACAACTTTCTGGAAAAACAGGATACGATGACTGACACTCAGA
  TGCCCAGTATCATACGTTGATAGTTTACAAACAAGATACGTATATTTTTAACTGCTTCAAGTGTTACCTA
  ACAATGTTTTTTATACTTCAAATGTCATTTCATACAAATTTTCCCAGTGAATAAATATTTTAGGAAACTC
  TCCATGATTATTAGTTGACCAACTATATTGCGAGAAACAGAGATCATAAAGAGCACGTTTTCCATTATGA
  GGAAACTTGGACATTTATGTACAAAATGAATTGCCTTTGATAATTCTTACTGTTCTGAAATTAGGAAAGT
  ACTGCATGATCTTACACGAAGAAATAGAATAGGCAAACTTTTATGCAGGCAGATTAATAACAGAAATACA
  TCATATGTTAGATACACAAAATATTTCCCTGAGGAAAAAAAATCAACTGCTTAAATTTTTTGGGAAGAAA
  AAGAAACATTTCTTACCCCACACCACCCACTACCTGAACAATAGCCAAGAAAGGAAAGGAGATGTTAAAA
  CAAACAGTGTTTTGATGACATTCTCAGCACTTGGCCCATGTGATCGCTGTCTCTGAAACACTGACCACCT
  CTCCCTACAGCCAGCGTGTAGTGCTTCAGTGGTGAGAAATTGTAATTGAGTTATTTTCCATTTTATTTCC
  TTGTATGTATTTCATGACTGGACTTACTGCTCTGTCAGCTTTTGTATATGAATCTTAAATGTTCATTAAA
  AAATAAAAAATGAACTGATCTTGTGGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001165037.1
• Summary: 'GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described. [provided by RefSeq, Jul 2008]'
• Locus ID: 2558