GATM (NM_001482) Human 3' UTR Clone

CAT#: SC211720

3`UTR clone of glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GATM
• Synonyms: AGAT; AT; CCDS3
• ACCN: NM_001482
• Insert Size: 1011 bp
• Sequence Data:
  Insert Sequence

  >SC211720 3'UTR clone of NM_001482
  The sequence shown below is from the reference sequence of NM_001482. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCACCTTACAGTCCTACTTGGACTGAACAGGCCTGATGGAGCTTGTGGCTGGCCTCAGATACACCTAA
  GAAGCTTAGGGGCAAGGTTCATTCTCCTGCTTTAAAAAGTGCATGAACTGTAGTGCTTTAAACAATCATC
  TCCTTAACAGGGGTCGTAAGCCTGGTTTGCTTCTATTACTTTTCTTTGACATAAAGAAAATAACTTCTGC
  TAGGTATTACTCTCTACTCCTAAAGTTATTTACTATTTGGCTTCAAGTATAAAATTTTGGTGAATGTGTA
  CCAAGAAAAAATTAGTCACCTGAGTAACTTGGCCACTAATAATTAACCATCTACCTCTGTTTTTAATTTT
  CTTTCCAAAAGGCAGCTTGAAATGTTGGTCCTAATCTTAATTTTTTTTCCTCTTCTATAGACTTGAGAAT
  GTTTTTCTCTAAATGAGAGAAAGACTTAGAATGTACACAGATCCAAAATAGAATCAGATTATCTCTTTTT
  TTCTAAAGGAGAGAAAGACTTAGAACATACACAGATCCTAAGTAGAACCAGGTAATTGTCTCTTTTTCTA
  ATAAGGAATTTGGGTAATTTTTAATTTTTTGTTTTTTAAAAAATAACCTAGACTATGCAAAACATCAAAG
  TGAATTTTCCATGAATGTTTTTAATATTCTCATCTCAACATTGTGATATATGCTACTAAAAACCTTTTCA
  TATACATCTTACCTCATTTCAAGTGAATTATTTTAATCTTTTTCTCTCTTTCCAAAAATTTAGGAATGTT
  TAGTGTAATTGGATTTCGCTATCAGTTCCCATCCTTAAGTTTTGATATTCAATATCTGATAGATACACTG
  CATCTTTGGTCATCTAAGATTTGTTTACAAATGTGCAAATTATTTAGAGCATAGACTTTATAAGCATTAA
  AAAAAACTAATGGAGGTAAAACCTAAATGCGATGTGAAATAATTTTAGTGTTGATACCGTATGTGTATTT
  TTATTCTAATAAACTTTTGTGTTCCAGATTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001482.2
• Summary: 'This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008]'
• Locus ID: 2628