CaV1.3 (CACNA1D) (NM_001128839) Human 3' UTR Clone

CAT#: SC212167

3`UTR clone of calcium channel voltage-dependent L type alpha 1D subunit (CACNA1D) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CACNA1D
• Synonyms: CACH3; CACN4; CACNL1A2; Cav1.3; CCHL1A2; PASNA; SANDD
• ACCN: NM_001128839
• Insert Size: 1076 bp
• Sequence Data:
  Insert Sequence

  >SC212167 3'UTR clone of NM_001128839
  The sequence shown below is from the reference sequence of NM_001128839. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCATCACCACCTTGTAGCCCCCAGCGAGGGGCAGACTGGCTCTGGCCTCAGGTGGGGCGCAGGAGAGCC
  AGGGGAAAAGTGCCTCATAGTTAGGAAAGTTTAGGCACTAGTTGGGAGTAATATTCAATTAATTAGACTT
  TTGTATAAGAGATGTCATGCCTCAAGAAAGCCATAAACCTGGTAGGAACAGGTCCCAAGCGGTTGAGCCT
  GGCAGAGTACCATGCGCTCGGCCCCAGCTGCAGGAAACAGCAGGCCCCGCCCTCTCACAGAGGATGGGTG
  AGGAGGCCAGACCTGCCCTGCCCCATTGTCCAGATGGGCACTGCTGTGGAGTCTGCTTCTCCCATGTACC
  AGGGCACCAGGCCCACCCAACTGAAGGCATGGCGGCGGGGTGCAGGGGAAAGTTAAAGGTGATGACGATC
  ATCACACCTGTGTCGTTACCTCAGCCATCGGTCTAGCATATCAGTCACTGGGCCCAACATATCCATTTTT
  AAACCCTTTCCCCCAAATACACTGCGTCCTGGTTCCTGTTTAGCTGTTCTGAAATACGGTGTGTAAGTAA
  GTCAGAACCCAGCTACCAGTGATTATTGCGAGGGCAATGGGACCTCATAAATAAGGTTTTCTGTGATGTG
  ACGCCAGTTTACATAAGAGAATATCACTCCGATGGTCGGTTTCTGACTGTCACGCTAAGGGCAACTGTAA
  ACTGGAATAATAATGCACTCGCAACCAGGTAAACTTAGATACACTAGTTTGTTTAAAATTATAGATTTAC
  TGTACATGACTTGTAATATACTATAATTTGTATTTGTAAAGAGATGGTCTATATTTTGTAATTACTGTAT
  TGTATTTGAACTGCAGCAATATCCATGGGTCCTAATAATTGTAGTTCCCCACTAAAATCTAGAAATTATT
  AGTATTTTTACTCGGGCTATCCAGAAGTAGAAGAAATAGAGCCAATTCTCATTTATTCAGCGAAAATCCT
  CTGGGGTTAAAATTTTAAGTTTGAAAGAACTTGACACTACAGAAATTTTTCTAAAATATTTTGAGTCACT
  ATAAACCTATCATCTTTCCACAAGAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001128839.1
• Summary: 'Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]'
• Locus ID: 776