MTHFD2 (NM_006636) Human 3' UTR Clone

CAT#: SC212261

3`UTR clone of methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 methenyltetrahydrofolate cyclohydrolase (MTHFD2) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MTHFD2
• Synonyms: NMDMC
• ACCN: NM_006636
• Insert Size: 1088
• Sequence Data:
  Insert Sequence

  >SC212261 3'UTR clone of NM_006636
  The sequence shown below is from the reference sequence of NM_006636. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCGAGAAGTGCTGAAGTCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCACAA
  ACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAATGCAATATTTTTAATTTATTCTACT
  GAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGGTGGGTGTTTCTGCACATACCTCT
  GCAGTACCTCACCAGGGAGCATTCCAGTATCATGCAGGGTCCTGTGATCTAGCCAGGAGCAGCCATTAAC
  CTAGTGATTAATATGGGAGACATTACCATATGGAGGATGGATGCTTCACTTTGTCAAGCACCTCAGTTAC
  ACATTCGCCTTTTCTAGGATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTAGGTAC
  CAAACCTTTTGAGTTCAACTGATCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGTGAA
  AAAGAAAAAATGGTAGTAATTGAGCAGAAAAAAATTAATTTATATATGTATTGATTGGCAACCAGATTTA
  TCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTAATCATTTTCCTAAGCTGTCCTTT
  TGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGATTATTCCTTGCTATTAGTACTCATTTTATGTATG
  TTACCCTTCAGTAAGTTCTCCCCATTTTAGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATG
  TGTGTTGTCATATTTGGCTTTTGCTATATACTTTAACTTCATTGTTAAATTTTTGTATTGTATAGTTTCT
  TTGGTGTATCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGTAAG
  TACGCAACTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATGTATGTATCCTGTT
  GACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTACTATTGAATTTAATCAGACTTTCTGATTAAAGGGTT
  TTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_006636.3
• Summary: This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
• Locus ID: 10797