ARFGAP3 (NM_001142293) Human 3' UTR Clone

CAT#: SC212294

3`UTR clone of ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARFGAP3
• Synonyms: ARFGAP1
• ACCN: NM_001142293
• Insert Size: 1107
• Sequence Data:
  Insert Sequence

  >SC212294 3'UTR clone of NM_001142293
  The sequence shown below is from the reference sequence of NM_001142293. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAATACTGAAGTCATGATGTGTATTTCC
  TGGAGAAATTCCTCTTTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCA
  GATTGTTTTGCTACTTTTTCATATGGTATATGTTTCTGATTTTTAATATTTCTTTTGAGAAATTCTGAGT
  TCTGATGTAGGAGCTTTCCTGTGATTTCTGTTTCACGTTCCTTCCTGTCACACCCTCCTTTGGCGTCTCT
  GTGTATATCCTTGCTTTATTTTCTTGGAACCTTTGATTTCAACACTGAGGGCCTGGAGACCTCGGCTCCT
  CCTGCTCCTGAACCAGGAGGCTTCATGTGGGGGAGGAGGAGAGGTCTCCATGTGACACATGGGCTCAGGG
  CTGCCAGAATCAGCGGATGCTGGATGGGCCTGCAGAAACAACACTCACCACACACACTTCCTTCAAAAGA
  CCAAAAGTGACTGGTGTCTCGTGTGACAGATTGCTTCATTTATGTTTCTACATAGTAAGGTGACTGCCAA
  ATAATATTTGAAGTCATCTGTCTCTTTGTAAATTATTTTATATGACCTATAAATTTAAAAATGTTTTTCA
  GTGAGTGCTTTTAACAAACTTAAGCTTCTGCCCTGCCAAGGGAATTAATGTTATCTTGTGAAAGGTGTTG
  CTGTTTGAATTGATGAGAAATGGAAGATGAGAACTCCCTAAGAGTTCTCATAATAAATCATCTCATCACA
  AATCAATACGGTATACAGAGTTAAAGTGGAATGAGGTAAGAAGATACAGCTACAGAAAATAGTTGCGTGT
  ATGGGAGAACAGTCATTGTAATTGGGTAGTTTTGTTAATAAATATTTTTAAATCTTGCTTTTCAGAAATT
  ACCGAATGTGTATAAACAAATAAAGAAAAATAATTTAGCTGTGTTTTAGACAGCATTAGAATATATTGTT
  CAGCACAGTAAAATATATTTGAAATTTGATAAGCCAAAAATGTGGTTTTGAATGAATATTTTGTGAATCT
  TTCTTAAAAGCTCAAATTTGTAGACTTCTAAATAGAATAAACACTTGCAGCAGATGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001142293.1
• Summary: The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
• Locus ID: 26286