Alkaline Phosphatase (ALPP) (NM_001632) Human 3' UTR Clone

CAT#: SC212497

3`UTR clone of alkaline phosphatase placental (Regan isozyme) (ALPP) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ALPP
• Synonyms: ALP; ALPI; IAP; PALP; PLAP; PLAP-1
• ACCN: NM_001632
• Insert Size: 1115 bp
• Sequence Data:
  Insert Sequence

  >SC212497 3'UTR clone of NM_001632
  The sequence shown below is from the reference sequence of NM_001632. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGAGACGGCCACTGCTCCCTGAGTGTCCCGTCCCTGGGGCTCCTGCTTCCCCATCCCGGAGTTCTCCTG
  CTCCCCACCTCCTGTCGTCCTGCCTGGCCTCCAGCCCGAGTCGTCATCCCCGGAGTCCCTATACAGAGGT
  CCTGCCATGGAACCTTCCCCTCCCCGTGCGCTCTGGGGACTGAGCCCATGACACCAAACCTGCCCCTTGG
  CTGCTCTCGGACTCCCTACCCCAACCCCAGGGACTGCAGGTTGTGCCCTGTGGCTGCCTGCACCCCAGGA
  AAGGAGGGGGCTCAGGCCATCCAGCCACCACCTACAGCCCAGTGGGTACCAGGCAGGCTCCCTTCCTGGG
  GAAAAGAAGCACCCAGACCCCGCGCCCCGCTGATCTTTGCTTCAGTCCTTGAATCACCTGTGGGACTTGA
  GGACTCGGGATCTTCAGGACGCCTGGAGAAGGGTGGTTTCCTGCCACCCTGCTGGCCAAGGAGGCTCCTG
  GGGTGGGGATCACCAGGGGGATTTTGACACAGCCTTCGGCTGCCCCCCACTAAGCTAATTCCACACCCCT
  GTACCCCCCCAGGGGGCCCTCTGCCTCATGGCAAAGGCTTGCCCCAAATCTCAACTTCTCAGACGTTCCA
  TACCCCCACATGCCAATTTCAGCACCCAACTGAGATCCGAGGAGCTCCTGGGAAGCCCTGGGTGCAGGAC
  ACTGGTCGAGAGCCAAAGGTCCCTCCCCAGACATCTGGACACTGGGCATAGATTTCTCAAGAAGGAAGAC
  TCCCCTGCCTCCCCAGGGCCTCTGCTCTCCTGGGAGACAAAGCAATAATAAAAGGAAGTGTTTGTAATCC
  CAGCACTTTGGGAGGCCGAGGTGGGCGGATCACGAGGTCAGGAGATGGAGACCATCCTGGCTAACACGGT
  GAAACCCCTTATCTATGCGCCTGTAGTCCCAGCTACCCAGGAGGCTGAAGCAGGATAATCGCTTGAACCC
  GGGCGGCGGAGATTGCAGTGAGCCGAGGTCATGCCACTGCACTGCAGCCTGGGCGACAGAGCGAGATTCT
  GCCTCAAAAATAAACAAATAAATTTTAAAAATAAATAAATAATAAAAGGAAGTGTTAGACAATGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001632.3
• Summary: 'The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]'
• Locus ID: 250