Nck beta (NCK2) (NM_001004720) Human 3' UTR Clone

CAT#: SC212557

3`UTR clone of NCK adaptor protein 2 (NCK2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NCK2
• Synonyms: GRB4; NCKbeta
• ACCN: NM_001004720
• Insert Size: 1099
• Sequence Data:
  Insert Sequence

  >SC212557 3'UTR clone of NM_001004720
  The sequence shown below is from the reference sequence of NM_001004720. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGAAGCTCTACCTCGTCAGGGCCCTGCAGTGACGGCGCCCCGGCCCCACACTCGCCTCCCGGGCCCCAC
  GGTGGAGCTGCCCGCCCGGCCTTGTGGCAGAGGCTCCTCCCGCGGGGACGGCCCCGACGGCTTCTCTGCG
  AGTCTCTCTTTATGTTCAGGTCGCTTGGTCGGTTCGTCTCCCATTTGCCATCCAGGCCTCACACCCACAC
  TCGAGCCCACCCGGCCGGCCAGCTTTAGAGGAGGGGAGGAGCAGGGCGAGTTCACATTATTCCTTTTCCA
  TCGGAAGTGGCGCTCGTGCATTCAACTCGTTCCCGCTCATGGAACCCCTCTTTAAAAAGACGCAGGGCAC
  CTGTGAGCGCAGGAGCGAGCCTAAGGCCACCCAGCGGCAGCGCCCGTGTCCTGGGCACTCAGCGTGCTGG
  GCAGAGCAGGTGCGATGGCCCCAGTCCTAGCAGCCCTCGCCCATGTCCTGTGCCCTTACATGGCTCCCGG
  ACTGTGCAGGGAGCCGATACGTTTGCTGATAGCAATACTGGAACCACCGGGTGCGATGGCAGTGAGGAGA
  CTGCCCAGTGCCTTTGGGGCTGTGCTTGCAATAAAGAATTTCCTGGAAAGGCAGTCTGCAAAAGAGGGAA
  CCGGTGACTCAGAAAGACAGATGTTTTGGTAATTTACCCCAAATGTGCCATCCACATAGTGCTTTTTCCT
  CTTGCCCTTCGGCTTGTTTGAATCTCACAATTATGTATTTAATTCTCAAAGAAATATGTATCTGTAGCCG
  TTTGTTGACACTAATACAGATGATTAAGGAAAACAGCTGATCTTTGGGGAAGGGAGCTACCAACACTTTA
  TACACACACACACGTGCACACACACACACACACACACTATATATATATATATTATTTACAGGGAAATTTT
  TCAGGGTTTACAAAAGAGTATGTGATTGGTAGTAAGAGACACACAGAATGTTTATGAAGAAATTGCATTT
  TCTTTTTCCTTTACATTTGAACTTCTTTATAGTTTAAATATAACGTCTTGAGATGGCACATTCCTACGAT
  TGAAGAAGGGGTCTTGAGATCCCCTAAACTTGCATACCCAGTTTTTTGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001004720.1
• Summary: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 8440