ROC2 (RNF7) (NM_014245) Human 3' UTR Clone

CAT#: SC212600

3`UTR clone of ring finger protein 7 (RNF7) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RNF7
• Synonyms: CKBBP1; rbx2; ROC2; SAG
• ACCN: NM_014245
• Insert Size: 1147
• Sequence Data:
  Insert Sequence

  >SC212600 3'UTR clone of NM_014245
  The sequence shown below is from the reference sequence of NM_014245. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGGTCCAAAGAATCGGCAAATGAGAGTGGTTAGAAGGCTTCTTAGCGCAGTTGTTCAGAGCCCTGGTGG
  ATCTTGTAATCCAGTGCCCTACAAAGGCTAGAACACTACAGGGGATGAATTCTTCAAATAGGAGCCGATG
  GATCTGTGGTCCTTTGGGACTCATCAAAGCCTTGGTTTAGCATTTTGTCAGTTTTATCTTCAGAAATTCT
  CTGCGATTAAGAAGATAATTTATTAAAGGTGGTCCTTCCTACCTCTGTGGTGTGTGTCGCGCACACAGCT
  TAGAAGTGCTATAAAAAAGGAAAGAGCTCCAAATTGAATCACCTTTATAATTTACCCATTTCTATACAAC
  AGGCAGTGGAAGCAGTTTCAGAGAACTTTTTGCATGCTTATGGTTGATCAGTTAAAAAAGAATGTTACAG
  TAACAAATAAAGTGCAGTTTAAAACCCAACTCTTACTCTTAATTTGTTCCTAATACGTATTTTTGGCAGG
  GAGAGGGAACGGTCCATGAAATCTTTATGTGATATAAGGATTTTAAGTTTGGGCCAGTGAACAGGGTAAA
  TAAAATTTAACTTTTGAGCATATGGAATTTTGATTGCCTTTAAAGTTACTATTCTGTATCATTGATTCAT
  CAAGAAAACCTAGATCTGCACTCACCTCACTGTTCAATCATTGAGTGGTAAAGGACAGAAGTATTTTCTA
  GTTCTGGTCAGATGATATAATTTTTTTTTTTTTTTTTTGTGGGGGTGAAGTCTTGCTCTGCCACCCAGGC
  TGGAGTGCAGTGGCATGATGTCAACTCACTGCAACCTCCGCCTCCCGGGTTCAAGTGATTCTCCTGCCTC
  AGCCTCCCGAGTAGCTGGGATTACAGGTGTGTGCCACCACACCCGGCTAATTTTTGTATTTTTAGTAGAG
  ACGGGATTTCCCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATTCGCCCACCTCAGCCT
  CCCAAAGTGCTGGGATTACAGGTGTGGGCCACTGCACCTGGCCCAGATGATATAATTTAACTGTGTTTTA
  GGTAACATGTTAATGAGGGAAAATGTTTACCAGTGTAGCATTGGATCAAAAAGTTTATCACACAGTGTTT
  TAATAAATGGTTTATTCTGCTTACCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_014245.3
• Summary: The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Locus ID: 9616