CIITA (NM_000246) Human 3' UTR Clone

CAT#: SC212637

3`UTR clone of class II major histocompatibility complex transactivator (CIITA) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CIITA
• Synonyms: C2TA; CIITAIV; MHC2TA; NLRA
• ACCN: NM_000246
• Insert Size: 1131 bp
• Sequence Data:
  Insert Sequence

  >SC212637 3'UTR clone of NM_000246
  The sequence shown below is from the reference sequence of NM_000246. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GATTCACGGATCAGCCTGAGATGATCCCAGCTGTGCTCTGGACAGGCATGTTCTCTGAGGACACTAACCA
  CGCTGGACCTTGAACTGGGTACTTGTGGACACAGCTCTTCTCCAGGCTGTATCCCATGAGCCTCAGCATC
  CTGGCACCCGGCCCCTGCTGGTTCAGGGTTGGCCCCTGCCCGGCTGCGGAATGAACCACATCTTGCTCTG
  CTGACAGACACAGGCCCGGCTCCAGGCTCCTTTAGCGCCCAGTTGGGTGGATGCCTGGTGGCAGCTGCGG
  TCCACCCAGGAGCCCCGAGGCCTTCTCTGAAGGACATTGCGGACAGCCACGGCCAGGCCAGAGGGAGTGA
  CAGAGGCAGCCCCATTCTGCCTGCCCAGGCCCCTGCCACCCTGGGGAGAAAGTACTTCTTTTTTTTTATT
  TTTAGACAGAGTCTCACTGTTGCCCAGGCTGGCGTGCAGTGGTGCGATCTGGGTTCACTGCAACCTCCGC
  CTCTTGGGTTCAAGCGATTCTTCTGCTTCAGCCTCCCGAGTAGCTGGGACTACAGGCACCCACCATCATG
  TCTGGCTAATTTTTCATTTTTAGTAGAGACAGGGTTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCTTG
  ACCTCAGGTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGATTACAAGCGTGAGCCACTGCACCGGGC
  CACAGAGAAAGTACTTCTCCACCCTGCTCTCCGACCAGACACCTTGACAGGGCACACCGGGCACTCAGAA
  GACACTGATGGGCAACCCCCAGCCTGCTAATTCCCCAGATTGCAACAGGCTGGGCTTCAGTGGCAGCTGC
  TTTTGTCTATGGGACTCAATGCACTGACATTGTTGGCCAAAGCCAAAGCTAGGCCTGGCCAGATGCACCA
  GCCCTTAGCAGGGAAACAGCTAATGGGACACTAATGGGGCGGTGAGAGGGGAACAGACTGGAAGCACAGC
  TTCATTTCCTGTGTCTTTTTTCACTACATTATAAATGTCTCTTTAATGTCACAGGCAGGTCCAGGGTTTG
  AGTTCATACCCTGTTACCATTTTGGGGTACCCACTGCTCTGGTTATCTAATATGTAACAAGCCACCCCAA
  ATCATAGTGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000246.3
• Summary: This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
• Locus ID: 4261