GRK2 (NM_001619) Human 3' UTR Clone

CAT#: SC212743

3`UTR clone of adrenergic beta receptor kinase 1 (ADRBK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GRK2
• Synonyms: ADRBK1; BARK1; BETA-ARK1
• ACCN: NM_001619
• Insert Size: 1126 bp
• Sequence Data:
  Insert Sequence

  >SC212743 3'UTR clone of NM_001619
  The sequence shown below is from the reference sequence of NM_001619. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAGGTGCCGCTGGTCCAGCGCGGCAGTGCCAACGGCCTCTGACCCGCCCACCCGCCTTTTATAAACCTCT
  AATTTATTTTGTCGAATTTTTATTATTTGTTTTCCCGCCAAGCGGAAAAGGTTTTATTTTGTAATTATTG
  TGATTTCCCGTGGCCCCAGCCTGGCCCAGCTCCCCCGGGAGGGGCCCGCTTGCCTCGGCTCCTGCTGCAC
  CAACCCAGCCGCTGCCCGGCGCCCTCTGTCCTGACTTCAGGGGCTGCCCGCTCCCAGTGTCTTCCTGTGG
  GGGAAGAGCACAGCCCTCCCGCCCCTTCCCCGAGGGATGATGCCACACCAAGCTGTGCCACCCTGGGCTC
  TGTGGGCTGCACTCTGTGCCCATGGGCACTGCTGGGTGGCCCATCCCCCCTCACCAGGGGCAGGCACAGC
  ACAGGGATCCGACTTGAATTTTCCCACTGCACCCCCTCCTGCTGCAGAGGGGCAGGCCCTGCACTGTCCT
  GCTCCACAGTGTTGGCGAGAGGAGGGGCCCGTTGTCTCCCTGGCCCTCAAGGCCTCCCACAGTGACTCGG
  GCTCCTGTGCCCTTATTCAGGAAAAGCCTCTGTGTCACTGGCTGCCTCCACTCCCACTTCCCTGACACTG
  CGGGGCTTGGCTGAGAGAGTGGCATTGGCAGCAGGTGCTGCTACCCTCCCTGCTGTCCCCTCTTGCCCCA
  ACCCCCAGCACCCGGGCTCAGGGACCACAGCAAGGCACCTGCAGGTTGGGCCATACTGGCCTCGCCTGGC
  CTGAGGTCTCGCTGATGCTGGGCTGGGTGCGACCCCATCTGCCCAGGACGGGGCCGGCCAGGTGGGCGGG
  CAGCACAGCAAGGAGGCTGGCTGGGGCCTATCAGTGTGCCCCCCATCCTGGCCCATCAGTGTACCCCCGC
  CCAGGCTGGCCAGCCCCACAGCCCACGTCCTGTCAGTGCCGCCGCCTCGCCCACCGCATGCCCCCTCGTG
  CCAGTCGCGCTGCCTGTGTGGTGTCGCGCCTTCTCCCCCCCGGGGCTGGGTTGGCGCACCCTCCCCTCCC
  GTCTACTCATTCCCCGGGGCGTTTCTTTGCCGATTTTTGAATGTGATTTTAAAGAGTGAAAAATGAGACT
  ATGCGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001619.3
• Summary: 'This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]'
• Locus ID: 156