STAT6 (NM_003153) Human 3' UTR Clone

CAT#: SC212972

3`UTR clone of signal transducer and activator of transcription 6 interleukin-4 induced (STAT6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: STAT6
• Synonyms: D12S1644; IL-4-STAT; STAT6B; STAT6C
• ACCN: NM_003153
• Insert Size: 1197 bp
• Sequence Data:
  Insert Sequence

  >SC212972 3'UTR clone of NM_003153
  The sequence shown below is from the reference sequence of NM_003153. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTCCCACATGGACCTAAGGGCCAACCCCAGTTGGTGATCCCAGCTGGAGGGAGAACCCAAAGAGACAGCT
  CTTCTACTACCCCCACAGACCTGCTCTGGACACTTGCTCATGCCCTGCCAAGCAGCAGATGGGGAGGGTG
  CCCTCCTATCCCCACCTACTCCTGGGTCAGGAGGAAAAGACTAACAGGAGAATGCACAGTGGGTGGAGCC
  AATCCACTCCTTCCTTTCTATCATTCCCCTGCCCACCTCCTTCCAGCACTGACTGGAAGGGAAGTTCAGG
  CTCTGAGACACGCCCCAACATGCCTGCACCTGCAGCGCGCACACGCACGCACACACACATACAGAGCTCT
  CTGAGGGTGATGGGGCTGAGCAGGAGGGGGGCTGGGTAAGAGCACAGGTTAGGGCATGGAAGGCTTCTCC
  GCCCATTCTGACCCAGGGCCTAGGACGGATAGGCAGGAACATACAGACACATTTACACTAGAGGCCAGGG
  ATAGAGGATATTGGGTCTCAGCCCTAGGGGAATGGGAAGCAGCTCAAGGGACCCTGGGTGGGAGCATAGG
  AGGGGTCTGGACATGTGGTTACTAGTACAGGTTTTGCCCTGATTAAAAAATCTCCCAAAGCCCCAAATTC
  CTGTTAGCCAGGTGGAGGCTTCTGATACGTGTATGAGACTATGCAAAAGTACAAGGGCTGAGATTCTTCG
  TGTATAGCTGTGTGAACGTGTATGTACCTAGGATATGTTAAATGTATAGCTGGCACCTTAGTTGCATGAC
  CACATAGAACATGTGTCTATCTGCTTTTGCCTACGTGACAACACAAATTTGGGAGGGTGAGACACTGCAC
  AGAAGACAGCAGCAAGTGTGCTGGCCTCTCTGACATATGCTAACCCCCAAATACTCTGAATTTGGAGTCT
  GACTGTGCCCAAGTGGGTCCAAGTGGCTGTGACATCTACGTATGGCTCCACACCTCCAATGCTGCCTGGG
  AGCCAGGGTGAGAGTCTGGGTCCAGGCCTGGCCATGTGGCCCTCCAGTGTATGAGAGGGCCCTGCCTGCT
  GCATCTTTTCTGTTGCCCCATCCACCGCCAGCTTCCCTTCACTCCCCTATCCCATTCTCCCTCTCAAGGC
  AGGGGTCATAGATCCTAAGCCATAAAATAAATTTTATTCCAAAATAACAAAATAAATAATCTACTGTACA
  CAATCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003153.3
• Summary: 'The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
• Locus ID: 6778