CD162 (SELPLG) (NM_003006) Human 3' UTR Clone

CAT#: SC212994

3`UTR clone of selectin P ligand (SELPLG) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SELPLG
• Synonyms: CD162; CLA; PSGL-1; PSGL1
• ACCN: NM_003006
• Insert Size: 1156 bp
• Sequence Data:
  Insert Sequence

  >SC212994 3'UTR clone of NM_003006
  The sequence shown below is from the reference sequence of NM_003006. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCACAGCTTCCTCCCTTAGCTCACTCTGCCATCTGTTTTGGCAAGACCCCACCTCCACGGGCTCTCCTG
  GGCCACCCCTGAGTGCCCAGACCCCATTCCACAGCTCTGGGCTTCCTCGGAGACCCCTGGGGATGGGGAT
  CTTCAGGGAAGGAACTCTGGCCACCCAAACAGGACAAGAGCAGCCTGGGGCCAAGCAGACGGGCAAGTGG
  AGCCACCTCTTTCCTCCCTCCGCGGATGAAGCCCAGCCACATTTCAGCCGAGGTCCAAGGCAGGAGGCCA
  TTTACTTGAGACAGATTCTCTCCTTTTTCCTGTCCCCCATCTTCTCTGGGTCCCTCTAACATCTCCCATG
  GCTCTCCCCGCTTCTCCTGGTCACTGGAGTCTCCTCCCCATGTACCCAAGGAAGATGGAGCTCCCCCATC
  CCACACGCACTGCACTGCCATTGTCTTTTGGTTGCCATGGTCACCAAACAGGAAGTGGACATTCTAAGGG
  AGGAGTACTGAAGAGTGACGGACTTCTGAGGCTGTTTCCTGCTGCTCCTCTGACTTGGGGCAGCTTGGGT
  CTTCTTGGGCACCTCTCTGGGAAAACCCAGGGTGAGGTTCAGCCTGTGAGGGCTGGGATGGGTTTCGTGG
  GCCCAAGGGCAGACCTTTCTTTGGGACTGTGTGGACCAAGGAGCTTCCATCTAGTGACAAGTGACCCCCA
  GCTATCGCCTCTTGCCTTCCCCTGTGGCCACTTTCCAGGGTGGACTCTGTCTTGTTCACTGCAGTATCCC
  AACTGCAGGTCCAGTGCAGGCAATAAATATGTGATGGACAAACGATAGCGGAATCCTTCAAGGTTTCAAG
  GCTGTCTCCTTCAGGCAGCCTTCCCGGAATTCTCCATCCCTCAGTGCAGGATGGGGGCTGGTCCTCAGCT
  GTCTGCCCTCAGCCCCTGGCCCCCCAGGAAGCCTCTTTCATGGGCTGTTAGGTTGACTTCAGTTTTGCCT
  CTTGGACAACAGGGGGTCTTGTACATCCTTGGGTGACCAGGAAAAGTTCAGGCTATGGGGGGCCAAAGGG
  AGGGCTGCCCCTTCCCCACCAGTGACCACTTTATTCCACTTCCTCCATTACCCAGTTTTGGCCCACAGAG
  TTTGGTCCCCCCCAAACCTCGGACCAATATCCCTCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003006.3
• Summary: 'This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]'
• Locus ID: 6404