ASCL1 (NM_004316) Human 3' UTR Clone

CAT#: SC213195

3`UTR clone of achaete-scute complex homolog 1 (Drosophila) (ASCL1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ASCL1
• Synonyms: ASH1; bHLHa46; HASH1; MASH1
• ACCN: NM_004316
• Insert Size: 1203 bp
• Sequence Data:
  Insert Sequence

  >SC213195 3'UTR clone of NM_004316
  The sequence shown below is from the reference sequence of NM_004316. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTCACCAACTGGTTCTGAGGGGCTCGGCCTGGTCAGGCCCTGGTGCGAATGGACTTTGGAAGCAGGGTG
  ATCGCACAACCTGCATCTTTAGTGCTTTCTTGTCAGTGGCGTTGGGAGGGGGAGAAAAGGAAAAGAAAAA
  AAAAAGAAGAAGAAGAAGAAAAGAGAAGAAGAAAAAAACGAAAACAGTCAACCAACCCCATCGCCAACTA
  AGCGAGGCATGCCTGAGAGACATGGCTTTCAGAAAACGGGAAGCGCTCAGAACAGTATCTTTGCACTCCA
  ATCATTCACGGAGATATGAAGAGCAACTGGGACCTGAGTCAATGCGCAAAATGCAGCTTGTGTGCAAAAG
  CAGTGGGCTCCTGGCAGAAGGGAGCAGCACACGCGTTATAGTAACTCCCATCACCTCTAACACGCACAGC
  TGAAAGTTCTTGCTCGGGTCCCTTCACCTCCTCGCCCTTTCTTAAAGTGCAGTTCTTAGCCCTCTAGAAA
  CGAGTTGGTGTCTTTCGTCTCAGTAGCCCCCACCCCAATAAGCTGTAGACATTGGTTTACAGTGAAACTA
  TGCTATTCTCAGCCCTTTGAAACTCTGCTTCTCCTCCAGGGCCCGATTCCCAAACCCCATGGCTTCCCTC
  ACACTGTCTTTTCTACCATTTTCATTATAGAATGCTTCCAATCTTTTGTGAATTTTTTATTATAAAAAAT
  CTATTTGTATCTATCCTAACCAGTTCGGGGATATATTAAGATATTTTTGTACATAAGAGAGAAAGAGAGA
  GAAAAATTTATAGAAGTTTTGTACAAATGGTTTAAAATGTGTATATCTTGATACTTTAACATGTAATGCT
  ATTACCTCTGCATATTTTAGATGTGTAGTTCACCTTACAACTGCAATTTTCCCTATGTGGTTTTGTAAAG
  AACTCTCCTCATAGGTGAGATCAAGAGGCCACCAGTTGTACTTCAGCACCAATGTGTCTTACTTTATAGA
  AATGTTGTTAATGTATTAATGATGTTATTAAATACTGTTCAAGAAGAACAAAGTTTATGCAGCTACTGTC
  CAAACTCAAAGTGGCAGCCAGTTGGTTTTGATAGGTTGCCTTTTGGAGATTTCTATTACTGCCTTTTTTT
  TTCTTACTGTTTTATTACAAACTTACAAAAATATGTATAACCCTGTTTTATACAAACTAGTTTCGTAATA
  AAACTTTTTCCTT
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004316.3
• Summary: 'This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]'
• Locus ID: 429