PLA2G5 (NM_000929) Human 3' UTR Clone

CAT#: SC213327

3`UTR clone of phospholipase A2 group V (PLA2G5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLA2G5
• Synonyms: FRFB; GV-PLA2; hVPLA(2); PLA2-10
• ACCN: NM_000929
• Insert Size: 1202 bp
• Sequence Data:
  Insert Sequence

  >SC213327 3'UTR clone of NM_000929
  The sequence shown below is from the reference sequence of NM_000929. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAACATCCTCTGCTCCTAGGCCTCCCCAGCGAGCTCCTCCCAGACCAAGACTTTTGTTCTGTTTTTCTA
  CAACACAGAGTACTGACTCTGCCTGGTTCCTGAGAGAGGCTCCTAAGTCACAGACCTCAGTCTTTCTCGA
  AGCTTGGCGGACCCCCAGGGCCACACTGTACCCTCCAGCGAGTCCCAGGAGAGTGACTCTGGTCATAGGA
  CTTGGTAGGGTCCCAGGGTCCCTAGGCCTCCACTTCTGAGGGCAGCCCCTCTGGTGCCAAGAGCTCTCCT
  CCAACTCAGGGTTGGCTGTGTCTCTTTTCTTCTCTGAAGACAGCGTCCTGGCTCCAGTTGGAACACTTTC
  CTGAGATGCACTTACTTCTCAGCTTCTGCGATCAGATTATCATCACCACCACCCTCCAGAGAATTTTTAC
  GCAAGAAGAGCCAAATTGACTCTCTAAATCTGGTGTATGGGTATTAAATAAAATTCATTCTCAAGGCTAA
  TAAAAACCACATTGGCATTTTCCTCTGCTGTGGGGGATCGCTGGTGCCTCTTTCTCTGCCACTGGGGCAA
  TAAACCCAAAGATGTCTACATTATCTCCGAAACAGAAGGGAAGATTAGTAAATGCAGGGTTTTCTGGGAT
  GAGCTTCAGGCTTTCTCTTGGGCTAATTTTCTTACACCTTGGGGTCCTCTCCAGTATTGGGTCTCATTCT
  TCCTCGATGGGGTCAGGGAAAGATAACTGGTGATTATGCCAGCTTCAGCTTCCAGGCCAGAGAGGGTGGC
  ATTCAAATCCCAGTGCTGGCTTCTTCAGCTGTGTGGTCTTGGACCCGTTACTGAACCTCTTTGACTTTCA
  GTCTCTTTGAGAAATAAACTGTCTTGTTCCTTGCAATGTAAAATGAGACTTCTAAAGCCCACCTTGATGC
  TGATATGGAGAATGCTGAGGTTCTAGGATTTCACACAGCAGGAATTTTTTTTTAATAGGTGTCAGCTGTG
  GGGTTTATTTTTTACAAAGTAAGGACATTAAAAAAACCAACCCGTCTATCAATTCATAAAAGAAAGGATG
  TTCTGATACCAAGACTGAAAGAAGAAAGGATGTATTCCAAAACAAAGGAACATCCTTCCAAGAAAGGACC
  TATGGCTTCTTTATTCCGACATACCCCAAAATAACTGCATGATAAATAGGTCTATATTTAAAAAGCTCTA
  GTGTCGAATGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000929.2
• Summary: 'This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]'
• Locus ID: 5322