ALDOB (NM_000035) Human 3' UTR Clone

CAT#: SC213471

3`UTR clone of aldolase B fructose-bisphosphate (ALDOB) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ALDOB
• Synonyms: ALDB; ALDO2
• ACCN: NM_000035
• Insert Size: 1274 bp
• Sequence Data:
  Insert Sequence

  >SC213471 3'UTR clone of NM_000035
  The sequence shown below is from the reference sequence of NM_000035. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTCTTCACAGCCTGCTATACCTACTAGGGTCCAATGCCCGCCAGCCTAGCTCCAGTGCTTCTAGTAGGA
  GGGCTGAAAGGGAGCAACTTTTCCTCCAATCCTGGAAATTCGACACAATTAGATTTGAACTGCTGGAAAT
  ACAACACATGTTAAATCTTAAGTACAAGGGGGAAAAAATAAATCAGTTATTGAAACATAAAAATGAATAC
  CAAGGACCTGATCAAATTTCACACAGCAGTTTCCTTGCAACACTTTCAGCTCCCCATGCTCCAGAATACC
  CACCCAAGAAAATAATAGGCTTTAAAACAATATCGGCTCCTCATCCAAAGAACAACTGCTGATTGAAACA
  CCTCATTAGCTGAGTGTAGAGAAGTGCATCTTATGAAACAGTCTTAGCAGTGGTAGGTTGGGAAGGAGAT
  AGCTGCAACCAAAAAAGAAATAAATATTCTATAAACCTTCAGCTGCTATCGGGTTTCACTTTTCTGCTCT
  TGCTGTCCAAAGACTCAGTGTATTTCATTACTTTTGACTCTACTAGACATGACTGGGTTTCAACAGTAAA
  GGTCTTCAACTCTTGCTAGTCATTGGAATCAAGCCGCAAAATTTTAAAAACTGAGATGCTCAGGCCACAC
  CCCAGCTCAATTAAATCAGAAACCCTAGACTTGGGATCCTCTAACTATTAGATTTCTTAAAGCTCCCTCA
  GTAATTCCAATGTACAGTCAAGTTTGAGAACTACCAATCTAAATTTCAAGTTTGAGGGTATTTGAAAATT
  AAAGCCATTCACAATACGAAGCCAGCTAAAAATGTAGAATGATTTTGAGCAACTTGTGGAGTATAATAAG
  AGAATTAATGTGACTTCAATGCTTGGAGCATTCTTGTTCAAGTGGCCCAGGTTTGGTGAAACAGGACTAC
  CTTGTCATCTGCACGTCCAGGCATATTTCGTAGTTTTGCAGTAAATAATATTCACATAATGATACTGTAT
  TGACTTTCAATTTTCAGAATTAACCTATAGTTACAGCACTTAAGACAACCAGAGTTATAAAAGAGAATTT
  AAATATTATAACTTTGGACAATATAAAAGTGATGATTTAACTGACAGAAGCTAGGAAATATAAGGGGGAG
  GAGAAGTGGAAGAAAGCAAAGAGAGTCAGGAATACTACTTAAAACTGATGGGTTAAGAAATAGTGCTTTA
  ATTCTATTTAAGTAATAAAAGAAATGGATGTAAATCATAAAAATATATATCTAAAATTAAAATATTGATG
  GTAGTATGCTAAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000035.3
• Summary: 'Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]'
• Locus ID: 229