PAK1 (NM_002576) Human 3' UTR Clone

CAT#: SC213547

3`UTR clone of p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAK1
• Synonyms: IDDMSSD; PAKalpha
• ACCN: NM_002576
• Insert Size: 1285 bp
• Sequence Data:
  Insert Sequence

  >SC213547 3'UTR clone of NM_002576
  The sequence shown below is from the reference sequence of NM_002576. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCAGCTAAGGAGGCAACAAAGAACAATCACTAAAACCACACTCACCCCAGCCTCATTGTGCCAAGCCTT
  CTGTGAGATAAATGCACATTTCAGAAATTCCAACTCCTGATGCCCTCTTCTCCTTGCCTTGCTTCTCCCA
  TTTCCTGATCTAGCACTCCTCAAGACTTTGATCCTTGGAAACCGTGTGTCCAGCATTGAAGAGAACTGCA
  ACTGAATGACTAATCAGATGATGGCCATTTCTAAATAAGGAATTTCCTCCCAATTCATGGATATGAGGGT
  GGTTTATGATTAAGGGTTTATATAAATAAATGTTTCTAGTCTTCCGTGTGTCAAAATCCTCACCTCCTTC
  ATAACCATCTCCCACAATTAATTCTTGACTATATAAATTTATGGTTTGATAATATTATCAATTTGTAATC
  AATTGAGATTTCTTTAGTGCTTGCTTTTCTGTGACTCAACTGCCCAGACACCTCATTGTACTTGAAAACT
  GGAACAGCTTGGGAATGCCATGGGGTTTGATAATCTGCCAGGGACATGAAGAGGCTCAGCTTCCTGGACC
  ATGACTTTGGCTCAGCTGATCCTGACATGGGAGAACAACCACATTTTTCTTTGTGTGTGCTTCTAGCAGC
  TGTTCGGGAGGACCTTGACCCAATAGTGTTCCCATGCTGTTTCTTGTGAAATGCTCTCGGCTATGTAGCA
  GCTTTTGATTCCCTGCATACCCTAGGCTGCTGCCCCTATCCTGTCCCTTGTTTATAACATTGAGAGGTTT
  TCTAGGGCACATACTGAGTGAGAGCAGTGTTGAGAAGTCGGGGAAAATGGTGACTACTTTTAGAGCAAGG
  CTGGGCATCAGCACCTGTCCAGCTCTACTTGTGTGATGTTTCAGGAACTCAGCCCCTTTTTCTGCCTAGG
  ATAAGGAGCTGAAAGATTAACTTGGATCTTCTAATGGTCCAAATCTTTTGGTCACAATAAAGAGTCTCCA
  AATTAGAGACTGCATGTTAGTTCTGGATGGATTTGGTGGCCTGACATGATACCCTGCCAGCTGTGAGGGG
  ACCCCGTTTTTAAGATGCATGGCCAAGCTCTCTGCAAATGGAAATGCTTACACTGGGTGTTGGGGATGTT
  TGCTACCTCCTGCTATTTTTGTGGTTTTGGTTCTCCCACTATGGTAGGACCCCTGGCCAGCATTGTGGCT
  TGTCATGTCAGCCCCATTGACTACCTTCTCATGCTCTGAGGTACTACTGCCTCTGCAGCACAAATTTCTA
  TTTCTGTCAATAAAAGGAGATGAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002576.4
• Summary: 'This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]'
• Locus ID: 5058