PAK1 (NM_001128620) Human 3' UTR Clone

CAT#: SC213730

3`UTR clone of p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAK1
• Synonyms: IDDMSSD; PAKalpha
• ACCN: NM_001128620
• Insert Size: 1301 bp
• Sequence Data:
  Insert Sequence

  >SC213730 3'UTR clone of NM_001128620
  The sequence shown below is from the reference sequence of NM_001128620. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCACTCCACTGATTGCTGCAGCTAAGGAGGCAACAAAGAACAATCACTAAAACCACACTCACCCCAGCCT
  CATTGTGCCAAGCCTTCTGTGAGATAAATGCACATTTCAGAAATTCCAACTCCTGATGCCCTCTTCTCCT
  TGCCTTGCTTCTCCCATTTCCTGATCTAGCACTCCTCAAGACTTTGATCCTTGGAAACCGTGTGTCCAGC
  ATTGAAGAGAACTGCAACTGAATGACTAATCAGATGATGGCCATTTCTAAATAAGGAATTTCCTCCCAAT
  TCATGGATATGAGGGTGGTTTATGATTAAGGGTTTATATAAATAAATGTTTCTAGTCTTCCGTGTGTCAA
  AATCCTCACCTCCTTCATAACCATCTCCCACAATTAATTCTTGACTATATAAATTTATGGTTTGATAATA
  TTATCAATTTGTAATCAATTGAGATTTCTTTAGTGCTTGCTTTTCTGTGACTCAACTGCCCAGACACCTC
  ATTGTACTTGAAAACTGGAACAGCTTGGGAATGCCATGGGGTTTGATAATCTGCCAGGGACATGAAGAGG
  CTCAGCTTCCTGGACCATGACTTTGGCTCAGCTGATCCTGACATGGGAGAACAACCACATTTTTCTTTGT
  GTGTGCTTCTAGCAGCTGTTCGGGAGGACCTTGACCCAATAGTGTTCCCATGCTGTTTCTTGTGAAATGC
  TCTCGGCTATGTAGCAGCTTTTGATTCCCTGCATACCCTAGGCTGCTGCCCCTATCCTGTCCCTTGTTTA
  TAACATTGAGAGGTTTTCTAGGGCACATACTGAGTGAGAGCAGTGTTGAGAAGTCGGGGAAAATGGTGAC
  TACTTTTAGAGCAAGGCTGGGCATCAGCACCTGTCCAGCTCTACTTGTGTGATGTTTCAGGAACTCAGCC
  CCTTTTTCTGCCTAGGATAAGGAGCTGAAAGATTAACTTGGATCTTCTAATGGTCCAAATCTTTTGGTCA
  CAATAAAGAGTCTCCAAATTAGAGACTGCATGTTAGTTCTGGATGGATTTGGTGGCCTGACATGATACCC
  TGCCAGCTGTGAGGGGACCCCGTTTTTAAGATGCATGGCCAAGCTCTCTGCAAATGGAAATGCTTACACT
  GGGTGTTGGGGATGTTTGCTACCTCCTGCTATTTTTGTGGTTTTGGTTCTCCCACTATGGTAGGACCCCT
  GGCCAGCATTGTGGCTTGTCATGTCAGCCCCATTGACTACCTTCTCATGCTCTGAGGTACTACTGCCTCT
  GCAGCACAAATTTCTATTTCTGTCAATAAAAGGAGATGAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001128620.1
• Summary: 'This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]'
• Locus ID: 5058