DNMT3A (NM_022552) Human 3' UTR Clone

CAT#: SC213901

3`UTR clone of DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DNMT3A
• Synonyms: DNMT3A2; M.HsaIIIA; TBRS
• ACCN: NM_022552
• Insert Size: 1311 bp
• Sequence Data:
  Insert Sequence

  >SC213901 3'UTR clone of NM_022552
  The sequence shown below is from the reference sequence of NM_022552. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCTGAAGGAGTATTTTGCGTGTGTGTAAGGGACATGGGGGCAAACTGAGGTAGCGACACAAAGTTAAAC
  AAACAAACAAAAAACACAAAACATAATAAAACACCAAGAACATGAGGATGGAGAGAAGTATCAGCACCCA
  GAAGAGAAAAAGGAATTTAAAACAAAAACCACAGAGGCGGAAATACCGGAGGGCTTTGCCTTGCGAAAAG
  GGTTGGACATCATCTCCTGATTTTTCAATGTTATTCTTCAGTCCTATTTAAAAACAAAACCAAGCTCCCT
  TCCCTTCCTCCCCCTTCCCTTTTTTTTCGGTCAGACCTTTTATTTTCTACTCTTTTCAGAGGGGTTTTCT
  GTTTGTTTGGGTTTTGTTTCTTGCTGTGACTGAAACAAGAAGGTTATTGCAGCAAAAATCAGTAACAAAA
  AATAGTAACAATACCTTGCAGAGGAAAGGTGGGAGAGAGGAAAAAAGGAAATTCTATAGAAATCTATATA
  TTGGGTTGTTTTTTTTTTTGTTTTTTGTTTTTTTTTTTTGGGTTTTTTTTTTTACTATATATCTTTTTTT
  TGTTGTCTCTAGCCTGATCAGATAGGAGCACAAGCAGGGGACGGAAAGAGAGAGACACTCAGGCGGCAGC
  ATTCCCTCCCAGCCACTGAGCTGTCGTGCCAGCACCATTCCTGGTCACGCAAAACAGAACCCAGTTAGCA
  GCAGGGAGACGAGAACACCACACAAGACATTTTTCTACAGTATTTCAGGTGCCTACCACACAGGAAACCT
  TGAAGAAAATCAGTTTCTAGAAGCCGCTGTTACCTCTTGTTTACAGTTTATATATATATGATAGATATGA
  GATATATATATAAAAGGTACTGTTAACTACTGTACAACCCGACTTCATAATGGTGCTTTCAAACAGCGAG
  ATGAGTAAAAACATCAGCTTCCACGTTGCCTTCTGCGCAAAGGGTTTCACCAAGGATGGAGAAAGGGAGA
  CAGCTTGCAGATGGCGCGTTCTCACGGTGGGCTCTTCCCCTTGGTTTGTAACGAAGTGAAGGAGGAGAAC
  TTGGGAGCCAGGTTCTCCCTGCCAAAAAGGGGGCTAGATGAGGTGGTCGGGCCCGTGGACAGCTGAGAGT
  GGGATTCATCCAGACTCATGCAATAACCCTTTGATTGTTTTCTAAAAGGAGACTCCCTCGGCAAGATGGC
  AGAGGGTACGGAGTCTTCAGGCCCAGTTTCTCACTTTAGCCAATTCGAGGGCTCCTTGTGGTGGGATCAG
  AACTAATCCAGAGTGTGGGAAAGTGACAGTCAAAACCCCACCTGGAGCAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_022552.3
• Summary: 'CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]'
• Locus ID: 1788